NDLI: Preliminary Studies of Stand-Alone Lance Concepts for Nanoinjection of DNA

Content Provider	The American Society of Mechanical Engineers (ASME) Digital Collection
Author	Mark, H. Fernelius Nathan, C. Toone Brian, D. Jensen Sandra, H. Burnett Larry, L. Howell
Copyright Year	2011
Abstract	Nanoinjection is an innovative approach for the electromechanical injection of DNA into cells, in which DNA is electrically attracted to a lance, inserted into a cell, and repelled by reversing the electrical polarity. In previous work, the lance has been micromachined as part of an on-chip microelectromechanical system. This work investigates a Stand-Alone Lance concept, where the lance and other components are independent of a common substrate. The Stand-Alone Lance may make nanoinjection more accessible to researchers and be more compatible with lab equipment commonly available in transgenic facilities. Required parameters for the electrode are investigated using a mathematical computer model. Different materials and fabrication processes for the metal lance are also considered. Additional testing was performed using tungsten probes, including mock injections on mouse egg cells. Based upon the optimistic cell viability rate, it is recommended to further investigate the use of the Stand-Alone Lance to perform nanoinjections.
Starting Page	471
Ending Page	479
Page Count	9
File Format	PDF
ISBN	9780791854976
DOI	10.1115/IMECE2011-64026
Volume Number	Volume 11: Nano and Micro Materials, Devices and Systems; Microsystems Integration
Conference Proceedings	ASME 2011 International Mechanical Engineering Congress and Exposition
Language	English
Publisher Date	2011-11-11
Publisher Place	Denver, Colorado, USA
Access Restriction	Subscribed
Subject Keyword	Computers Electrodes Microelectromechanical systems Dna Metals Tungsten Micromachining Manufacturing Probes Testing
Content Type	Text
Resource Type	Article

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