NDLI: TLR3-triggered reactive oxygen species contribute to inflammatory responses by activating signal transducer and activator of transcription-1.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Hwang, Jung Hwan Lee, Sung Joong Kim, Jwa-Jin Jo, Eun-Kyeong Yang, Chul-Su Lee, Chul-Ho Lee, Myung-Shik
Description	Country affiliation: South Korea Author Affiliation: Yang CS ( Department of Microbiology, Chungnam National University, Daejeon 301-747, South Korea.)
Abstract	Intracellular reactive oxygen species (ROS) are essential secondary messengers in many signaling cascades governing innate immunity and cellular functions. TLR3 signaling is crucially involved in antiviral innate and inflammatory responses; however, the roles of ROS in TLR3 signaling remain largely unknown. In this study, we show that TLR3-induced ROS generation is required for the activation of NF-κB, IFN-regulatory factor 3, and STAT1-mediated innate immune responses in macrophages. TLR3 induction led to a rapid increase in ROS generation and a physical association between components of the NADPH oxidase (NOX) enzyme complex (NOX2 and p47(phox)) and TLR3 via a Ca(2+)-c-Src tyrosine kinase-dependent pathway. TLR3-induced ROS generation, NOX2, and p47(phox) were required for the phosphorylation and nuclear translocation of STAT1 and STAT2. TLR3-induced activation of STAT1 contributed to the generation of inflammatory mediators, which was significantly attenuated in NOX2- and p47(phox)-deficient macrophages, suggesting a role for ROS-STAT1 in TLR3-mediated innate immune responses. Collectively, these results provide a novel insight into the crucial role that TLR3-ROS signaling plays in innate immune responses by activating STAT1.
ISSN	00221767
e-ISSN	15506606
Journal	The Journal of Immunology
Issue Number	12
Volume Number	190
Language	English
Publisher	The American Association of Immunologists
Publisher Date	2013-06-15
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Immunity, Innate Immunology Reactive Oxygen Species Stat1 Transcription Factor Signal Transduction Toll-like Receptor 3 Animals Blotting, Western Cells, Cultured Fluorescent Antibody Technique Immunoprecipitation Inflammation Metabolism Macrophages Mice Mice, Inbred C57bl Mice, Knockout Microscopy, Confocal Reverse Transcriptase Polymerase Chain Reaction Research Support, Non-u.s. Gov't Discipline Immunology
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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