NDLI: Critical roles of a dendritic cell subset expressing a chemokine receptor, XCR1.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Hemmi, Hiroaki Kaisho, Tsuneyasu Hirashima, Takeshi Sasaki, Izumi Sato, Katsuaki Hoshino, Katsuaki Ohta, Tomokazu Sugiyama, Masanaka Hamada, Eri Yamazaki, Chihiro Yano, Takahiro Nobuoka, Mikako Fukuda, Yuri Tanaka, Takashi Iizuka, Akihiko
Description	Country affiliation: Japan Author Affiliation: Yamazaki C ( Laboratory for Host Defense, RIKEN Research Center for Allergy and Immunology, Yokohama, Kanagawa 230-0045, Japan.)
Abstract	Dendritic cells (DCs) consist of various subsets that play crucial roles in linking innate and adaptive immunity. In the murine spleen, CD8 (+) DCs exhibit a propensity to ingest dying/dead cells, produce proinflammatory cytokines, and cross-present Ags to generate CD8(+) T cell responses. To track and ablate CD8 (+) DCs in vivo, we generated XCR1-venus and XCR1-DTRvenus mice, in which genes for a fluorescent protein, venus, and a fusion protein consisting of diphtheria toxin receptor and venus were knocked into the gene locus of a chemokine receptor, XCR1, which is highly expressed in CD8 (+) DCs. In both mice, venus(+) cells were detected in the majority of CD8 (+) DCs, but they were not detected in any other cells, including splenic macrophages. Venus(+)CD8 (+) DCs were superior to venus(-)CD8 (+) DCs with regard to their cytokine-producing ability in response to TLR stimuli. In other tissues, venus(+) cells were found primarily in lymph node (LN)-resident CD8 (+), LN migratory and peripheral CD103(+) DCs, which are closely related to splenic CD8 (+) DCs, although some thymic CD8 (-)CD11b(-) and LN CD103(-)CD11b(-) DCs were also venus(+). In response to dsRNAs, diphtheria toxin-treated XCR1-DTR mice showed impaired CD8(+) T cell responses, with retained cytokine and augmented CD4(+) T cell responses. Furthermore, Listeria monocytogenes infection and anti-L. monocytogenes CD8(+) T cell responses were defective in diphtheria toxin-treated XCR1-DTRvenus mice. Thus, XCR1-expressing DCs were required for dsRNA- or bacteria-induced CD8(+) T cell responses. XCR1-venus and XCR1-DTRvenus mice should be useful for elucidating the functions and behavior of XCR1-expressing DCs, including CD8 (+) and CD103(+) DCs, in lymphoid and peripheral tissues.
ISSN	00221767
e-ISSN	15506606
Journal	The Journal of Immunology
Issue Number	12
Volume Number	190
Language	English
Publisher	The American Association of Immunologists
Publisher Date	2013-06-15
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Cross-priming Immunology Dendritic Cells Receptors, Chemokine Animals Antigen Presentation Bacterial Proteins Genetics Metabolism Cell Separation Flow Cytometry Gene Knock-in Techniques Luminescent Proteins Mice Mice, Inbred C57bl Research Support, Non-u.s. Gov't Discipline Immunology
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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