NDLI: Quantitative detection of viable helminth ova from raw wastewater, human feces, and environmental soil samples using novel PMA-qPCR methods.

Please wait, while we are loading the content...

Year: 2017 Volume: 24

Year: 2016 Volume: 23

Year: 2016 Volume: 23 Issue: 19

Year: 2016 Volume: 23 Issue: 18

Cadmium stress in rice: toxic effects, tolerance mechanisms, and management: a critical review.

Nanoscale zero-valent metals: a review of synthesis, characterization, and applications to environmental remediation.

Effects of organic acids on the photosynthetic and antioxidant properties and accumulations of heavy metals of Melilotus officinalis grown in Cu tailing.

Toxicity of Cu (II) to the green alga Chlorella vulgaris: a perspective of photosynthesis and oxidant stress.

Water reclamation during drinking water treatments using polyamide nanofiltration membranes on a pilot scale.

Effects of production conditions on yield and physicochemical properties of biochars produced from rice husk and oil palm empty fruit bunches.

Cadmium (Cd) distribution and contamination in Chinese paddy soils on national scale.

Trends and variations of pH and hardness in a typical semi-arid river in a monsoon climate region during 1985–2009

Molecular and phenotypic responses of Japanese medaka (Oryzias latipes) early life stages to environmental concentrations of cadmium in sediment.

Solar-driven photocatalytic treatment of diclofenac using immobilized TiO2-based zeolite composites.

Heavy metal and trace elements in riparian vegetation and macrophytes associated with lacustrine systems in Northern Patagonia Andean Range.

Evaluating levels and health risk of heavy metals in exposed workers from surgical instrument manufacturing industries of Sialkot, Pakistan.

Use of sugarcane filter cake and nitrogen, phosphorus and potassium fertilization in the process of bioremediation of soil contaminated with diesel

Adsorptive removal of naphthalene induced by structurally different Gemini surfactants in a soil-water system.

Suitability of passive sampling for the monitoring of pharmaceuticals in Finnish surface waters.

Distribution, fate, and risk assessment of antibiotics in five wastewater treatment plants in Shanghai, China.

Assessment of the individual and mixture toxicity of cadmium, copper and oxytetracycline, on the embryo-larval development of the sea urchin Paracentrotus lividus.

Integrated spatial health assessment of yellow perch (Perca flavescens) populations from the St. Lawrence River, Quebec, Canada) part A: physiological parameters and pathogen assessment.

Exploitation of subabul stem lignin as a matrix in controlled release agrochemical nanoformulations: a case study with herbicide diuron.

Impact of molybdenum nanoparticles on survival, activity of enzymes, and chemical elements in Eisenia fetida using test on artificial substrata.

Bioavailability of pollutants sets risk of exposure to biota and human population in reservoirs from Iguaçu River (Southern Brazil).

Surfactant enhanced pyrene degradation in the rhizosphere of tall fescue (Festuca arundinacea).

An improved biofilter to control the dissolved organic nitrogen concentration during drinking water treatment.

Insight into visible light-driven photocatalytic degradation of diesel oil by doped $TiO_{2}-PS$ floating composites

Microbial disinfection of water with endotoxin degradation by photocatalysis using Ag@TiO2 core shell nanoparticles.

Cultivating Chlorella vulgaris and Scenedesmus quadricauda microalgae to degrade inorganic compounds and pesticides in water.

Stable isotope on the evaluation of water quality in the presence of WWTPs in rivers

Combination of antibiotics suppressed the increase of a part of ARGs in fecal microorganism of weaned pigs.

Constructed wetlands may lower inorganic nutrient inputs but enhance DOC loadings into a drinking water reservoir in North Wales

Impact of arsenic(V) on testicular oxidative stress and sperm functional attributes in Swiss albino mice.

Integrated spatial health assessment of yellow perch (Perca flavescens) populations from the St. Lawrence River (QC, Canada), part B: cellular and transcriptomic effects.

Long-term effects of antibiotics, norfloxacin, and sulfamethoxazole, in a partial life-cycle study with zebrafish (Danio rerio): effects on growth, development, and reproduction.

Genotype-dependent effect of exogenous 24-epibrassinolide on chromium-induced changes in ultrastructure and physicochemical traits in tobacco seedlings.

Heavy metal bioaccumulation by wild edible saprophytic and ectomycorrhizal mushrooms.

Forest floor decomposition, metal exchangeability, and metal bioaccumulation by exotic earthworms: Amynthas agrestis and Lumbricus rubellus

BTX in urban areas of eastern Spain: a focus on time variations and sources.

Vinegar residue compost as a growth substrate enhances cucumber resistance against the Fusarium wilt pathogen Fusarium oxysporum by regulating physiological and biochemical responses.

Industrial-scale application of the plunger flow electro-oxidation reactor in wastewater depth treatment.

Effects and mechanism of acid rain on plant chloroplast ATP synthase

Novel bacterial consortia isolated from plastic garbage processing areas demonstrated enhanced degradation for low density polyethylene.

Influence of sub-lethal crude oil concentration on growth, water relations and photosynthetic capacity of maize (Zea mays L.) plants

Elemental composition of Marrubium astracanicum Jacq. growing in tungsten-contaminated sites

Acute toxicity and chemical evaluation of coking wastewater under biological and advanced physicochemical treatment processes.

Estimation of environmentally relevant chemical properties of veterinary ionophore antibiotics

Effects of Î³-Fe2O3 nanoparticles on the survival and reproduction of Biomphalaria glabrata (Say, 1818) and their elimination from this benthic aquatic snail.

$Ag/Ag_{2}SO_{3}$ plasmonic catalysts with high activity and stability for $CO_{2}$ reduction with water vapor under visible light

Biological responses of midge (Chironomus riparius) and lamprey (Lampetra fluviatilis) larvae in ecotoxicity assessment of PCDD/F-, PCB- and Hg-contaminated river sediments.

Response of leaf and fine roots proteomes of Salix viminalis L. to growth on Cr-rich tannery waste.

The environmental Kuznets curve and $CO_{2}$ emissions in the USA

Morphological effect of BiVO4 catalysts on degradation of aqueous paracetamol under visible light irradiation.

Phenol degradation by Fenton-like process

Improving of local ozone forecasting by integrated models.

Impact of northern and southern air mass transport on the temporal distribution of atmospheric (210)Po and (210)Pb in the east coast of Johor, Malaysia.

Response of the turbidity maximum zone in the Yangtze River Estuary due to human activities during the dry season.

Effect of vegetation types on soil arbuscular mycorrhizal fungi and nitrogen-fixing bacterial communities in a karst region.

Sorption and pH determine the long-term partitioning of cadmium in natural soils

Evaluation of atrazine degradation applied to different energy systems.

Estimating internal P loading in a deep water reservoir of northern China using three different methods.

The influence of humic substance on Cd accumulation of phytostabilizer Athyrium wardii (Hook.) grown in Cd-contaminated soils.

Occurrences and toxicological risk assessment of eight heavy metals in agricultural soils from Kenya, Eastern Africa.

Ce-Fe-reduced graphene oxide nanocomposite as an efficient catalyst for sulfamethazine degradation in aqueous solution

Application of TiO2-organobentonite modified by cetyltrimethylammonium chloride photocatalyst and polyaluminum chloride coagulant for pretreatment of aging landfill leachate.

Activated carbon fiber for heterogeneous activation of persulfate: implication for the decolorization of azo dye

Pollutant emissions and environmental assessment of ethyl 3-ethoxybutyrate, a potential renewable fuel.

Effect of different amendments on rice (Oryza sativa L.) growth, yield, nutrient uptake and grain quality in Ni-contaminated soil.

Phytotoxic effects of irrigation water depending on the presence of organic and inorganic pollutants.

Highly selective catalytic reduction of NO via $SO_{2}/H_{2}O-tolerant$ spinel catalysts at low temperature

Characteristics of metal-tolerant plant growth-promoting yeast (Cryptococcus sp. NSE1) and its influence on Cd hyperaccumulator Sedum plumbizincicola.

Associations of morbidity and mortality from coronary heart disease with heliogeophysical factors.

Quantitative detection of viable helminth ova from raw wastewater, human feces, and environmental soil samples using novel PMA-qPCR methods.

Enantioselective metabolism and toxic effects of metalaxyl on primary hepatocytes from rat.

Fast removal of tetracycline from wastewater by reduced graphene oxide prepared via microwave-assisted ethylenediamine-N,N'-disuccinic acid induction method.

Characterizing scale-specific environmental factors affecting soil organic carbon along two landscape transects.

From laboratory to environmental conditions: a new approach for chemical's biodegradability assessment.

Bioassays with terrestrial and aquatic species as monitoring tools of hydrocarbon degradation.

The toxicity of arsenic(III), chromium(VI) and zinc to groundwater copepods.

Blood cadmium in Chinese adults and its relationships with diabetes and obesity.

Reductive dechlorination in recalcitrant sources of chloroethenes in the transition zone between aquifers and aquitards.

Concentrations of and health risks posed by polychlorinated dibenzo-p-dioxins and dibenzofurans around industrial sites in Hebei Province, China.

Natural gas consumption, income, urbanization, and $CO_{2}$ emissions in China and India

Burrowing mayfly Ephemera orientalis (Ephemeroptera: Ephemeridae) as a new test species for pesticide toxicity.

Effects of chlorinated drinking water on the xenobiotic metabolism in Cyprinus carpio treated with samples from two Italian municipal networks.

Distribution and estrogenic potential of endocrine disrupting chemicals (EDCs) in estuarine sediments from Mumbai, India.

Fe-Mn bi-metallic oxides loaded on granular activated carbon to enhance dye removal by catalytic ozonation

Spatio-temporal variation in chemical characteristics of PM10 over Indo Gangetic Plain of India.

Morphophysiological characteristic analysis demonstrated the potential of sweet sorghum (Sorghum bicolor (L.) Moench) in the phytoremediation of cadmium-contaminated soils.

Acute toxicity and histopathological effects of naproxen in zebrafish (Danio rerio) early life stages.

Cadmium accumulation characteristics of F1 hybrids by reciprocal hybridizing of Solanum nigrum in two climate–ecology regions

Laccase encapsulation in chitosan nanoparticles enhances the protein stability against microbial degradation.

Resilience assessment of a biological early warning system based on the locomotor behavior of zebrafish (Danio rerio).

Differential impact of Limnoperna fortunei-herbicide interaction between Roundup Max® and glyphosate on freshwater microscopic communities.

Phosphorus adsorption on natural sediments with different pH incorporating surface morphology characterization.

Fish liver and gill cells as cytogenotoxic indicators in assessment of water quality.

Kinetics and equilibrium partitioning of dissolved BTEX in PDMS and POM sheets.

Hydrogeochemical features of surface water and groundwater contaminated with acid mine drainage (AMD) in coal mining areas: a case study in southern Brazil.

Speciation of potentially mobile Si in Yangtze Estuary surface sediments: estimates using a modified sequential extraction technique.

Discussion on: Dahab AA, Elhag DEA, Ahmed AB, Al-Obaid HA. Determination of elemental toxicity migration limits, bioaccessibility and risk assessment of essential childcare products. Environmental science and pollution research, 2016, 23, 3406-3413.

”LOVE TO HATE” pesticides: felicity or curse for the soil microbial community? An FP7 IAPP Marie Curie project aiming to establish tools for the assessment of the mechanisms controlling the interactions of pesticides with soil microorganisms

Awasthi, A. K., Zeng, X., & Li, J. (2016). Relationship between e-waste recycling and human health risk in India: a critical review. Environmental Science and Pollution Research, 1-24.

Year: 2016 Volume: 23 Issue: 17

Year: 2016 Volume: 23 Issue: 16

Year: 2016 Volume: 23 Issue: 15

Year: 2016 Volume: 23 Issue: 14

Year: 2016 Volume: 23 Issue: 13

Year: 2016 Volume: 23 Issue: 12

Year: 2016 Volume: 23 Issue: 11

Year: 2016 Volume: 23 Issue: 10

Year: 2016 Volume: 23 Issue: 9

Year: 2016 Volume: 23 Issue: 8

Year: 2016 Volume: 23 Issue: 7

Year: 2016 Volume: 23 Issue: 6

Year: 2016 Volume: 23 Issue: 5

Year: 2016 Volume: 23 Issue: 4

Year: 2016 Volume: 23 Issue: 3

Year: 2016 Volume: 23 Issue: 2

Year: 2016 Volume: 23 Issue: 1

Year: 2015 Volume: 22

Year: 2014 Volume: 21

Year: 2013 Volume: 20

Year: 2012 Volume: 19

Year: 2011 Volume: 19

Year: 2011 Volume: 18

Year: 2010 Volume: 17

Year: 2009 Volume: 16

Year: 2008 Volume: 15

Year: 2007 Volume: 14

Year: 2000 Volume: 7

Year: 1999 Volume: 6

Year: 1998 Volume: 5

Year: 1996 Volume: 3

Year: 1995 Volume: 2

Year: 1994 Volume: 1

Quantitative detection of viable helminth ova from raw wastewater, human feces, and environmental soil samples using novel PMA-qPCR methods.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Ahmed, W. McCarthy, J. S. Sidhu, J. P. S. Llewellyn, S. Traub, R. Jagals, P. Gyawali, P. Toze, S. Clements, A. C. Nery, S. V.
Description	Country affiliation: Australia Author Affiliation: Gyawali P ( CSIRO Land and Water, Ecosciences Precinct, 41 Boggo Road, GPO Box 2583, Brisbane, QLD, 4102, Australia. pradep033@yahoo.com.); Ahmed W ( School of Public Health, The University of Queensland, Herston, QLD, 4006, Australia. pradep033@yahoo.com.); Sidhu JP ( CSIRO Land and Water, Ecosciences Precinct, 41 Boggo Road, GPO Box 2583, Brisbane, QLD, 4102, Australia.); Nery SV ( CSIRO Land and Water, Ecosciences Precinct, 41 Boggo Road, GPO Box 2583, Brisbane, QLD, 4102, Australia.); Clements AC ( School of Public Health, The University of Queensland, Herston, QLD, 4006, Australia.); Traub R ( Research School of Population Health, College of Medicine, Biology and Environment, The Australian National University, Linnaeus Way, ACT, 2601, Australia.); McCarthy JS ( Research School of Population Health, College of Medicine, Biology and Environment, The Australian National University, Linnaeus Way, ACT, 2601, Australia.); Llewellyn S ( Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, 3052, Australia.); Jagals P ( Clinical Tropical Medicine Laboratory, QIMR Berghofer Medical Research Institute, Herston, QLD, 4006, Australia.); Toze S ( School of Medicine, The University of Queensland, Herston, QLD, 4006, Australia.)
Abstract	In this study, we have evaluated the efficacy of propidium monoazide quantitative polymerase chain reaction (PMA-qPCR) to differentiate between viable and non-viable Ancylostoma caninum ova. The newly developed method was validated using raw wastewater seeded with known numbers of A. caninum ova. Results of this study confirmed that PMA-qPCR has resulted in average of 88 % reduction (P < 0.05) in gene copy numbers for 50 % viable +50 % non-viable when compared with 100 % viable ova. A reduction of 100 % in gene copies was observed for 100 % non-viable ova when compared with 100 % viable ova. Similar reductions (79-80 %) in gene copies were observed for A. caninum ova-seeded raw wastewater samples (n = 18) collected from wastewater treatment plants (WWTPs) A and B. The newly developed PMA-qPCR method was applied to determine the viable ova of different helminths (A. caninum, A. duodenale, Necator americanus and Ascaris lumbricoides) in raw wastewater, human fecal and soil samples. None of the unseeded wastewater samples were positive for the above-mentioned helminths. N. americanus and A. lumbricoides ova were found in unseeded human fecal and soil samples. For the unseeded human fecal samples (1 g), an average gene copy concentration obtained from qPCR and PMA-qPCR was found to be similar (6.8 × 10(5) ± 6.4 × 10(5) and 6.3 × 10(5) ± 4.7 × 10(5)) indicating the presence of viable N. americanus ova. Among the 24 unseeded soil samples tested, only one was positive for A. lumbricoides. The mean gene copy concentration in the positively identified soil sample was 1.0 × 10(5) ± 1.5 × 10(4) (determined by qPCR) compared to 4.9 × 10(4) ± 3.7 × 10(3) (determined by PMA-qPCR). The newly developed PMA-qPCR methods were able to detect viable helminth ova from wastewater and soil samples and could be adapted for health risk assessment.
ISSN	09441344
Issue Number	18
Journal	Environmental Science and Pollution Research
Volume Number	23
e-ISSN	16147499
Language	English
Publisher	Springer
Publisher Date	2016-09-01
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Environmental Science Discipline Environmental Chemistry
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Health, Toxicology and Mutagenesis Pollution Medicine

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in