NDLI: Improving immunosensor performance through oriented immobilization of antibodies on carbon nanotube composite surfaces.

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Improving immunosensor performance through oriented immobilization of antibodies on carbon nanotube composite surfaces.

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Improving immunosensor performance through oriented immobilization of antibodies on carbon nanotube composite surfaces.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Puertas, Sara Grazú, Valeria Fernández-Sánchez, César Jiménez-Jorquera, Cecilia de Gracia Villa, María Mendoza, Ernest de la Fuente, Jesús M.
Description	Country affiliation: Spain Author Affiliation: Puertas S ( Biofunctionalization of Nanoparticles and Surfaces (BioNanoSurf), Instituto de Nanociencia de Aragón (INA), Universidad de Zaragoza. Campus Río Ebro, Edifício I+D, Mariano Esquillor, s/n, 50018 Zaragoza, Spain. selmer@unizar.es)
Abstract	We report the straightforward oriented covalent attachment of antibodies (Abs) on the surface of carboxylated multiwalled carbon nanotube-polystyrene (MWCNT-PS) materials. The combination of this composite material, applied as a robust electrochemical transducer platform, and its covalent functionalization with Abs in a controlled way by means of a two-step process, could contribute to the development of highly sensitive immunosensor devices. Using the simple and versatile carbodiimide chemistry, Abs were attached to the carboxylic groups of the MWCNT-PS composite surfaces via their superficial amine groups. By taking into account the Ab isoelectric point and the net charge of the composite surface, we engineered an immobilization process to achieve the oriented binding of the Ab molecules by favoring an ionic pre-adsorption step before covalent binding occurred. Thus, the antigen binding capacity of the attached Abs was enhanced by up to 10 times with respect to the capacity estimated for a random spatial distribution of these molecules. The proposed strategy would also serve as a model for the efficient biofunctionalization of other carboxylated carbon-based polymer composite materials with potential applications in the biosensor field.
ISSN	09565663
Volume Number	43
e-ISSN	18734235
Journal	Biosensors and Bioelectronics
Language	English
Publisher	Elsevier
Publisher Date	2013-05-15
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Antibodies Chemistry Biosensing Techniques Instrumentation Immunoassay Nanotubes, Carbon Ultrastructure Adsorption Binding Sites Equipment Design Equipment Failure Analysis Protein Binding Surface Properties Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry

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