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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Guo, Lei Tang, Jijun Xie, Jianwei Sun, Jiefang Xu, Hua |
| Description | Country affiliation: China Author Affiliation: Sun J ( Beijing Institute of Pharmacology and Toxicology, No. 27, Taiping Road, Beijing 100850, China.) |
| Abstract | In this paper, denatured bovine serum albumin (dBSA) has been developed as a useful scaffold for appending multiple oligonucleotides (ODNs), and the resulted novel dBSA-ODNs covalent hybrid can be self-assembled to the surface of CdTe quantum dots (QDs) via complexation with unoccupied coordination metal sites on QDs' surface. The formed QDs/dBSA-ODNs bioconjugate could keep a long-term stability in aqueous solution while maintaining its fluorescence quantum yield and spectral properties. Furthermore, the QDs/dBSA-ODNs also can be readily hybridized with an anti-rHuEPO- aptamers linked AuNPs (Apt-AuNPs) to form a fluorescent resonance energy transfer-based nano-biosensing system for highly sensitive and selective detection of rHuEPO- protein lowered to 53.6 pM in a rapid, simple manner. |
| ISSN | 09565663 |
| Volume Number | 43 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-05-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Aptamers, Nucleotide Chemistry Erythropoietin Analysis Gold Metal Nanoparticles Quantum Dots Serum Albumin, Bovine Spectrometry, Fluorescence Instrumentation Crystallization Methods Equipment Design Equipment Failure Analysis Humans Oligonucleotides Protein Denaturation Recombinant Proteins Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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