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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Torrents, Eduard Jaramillo, Maria Del Carmen Hüttener, Mario Juárez, Antonio Martínez-Duarte, Rodrigo Renaud, Philippe |
| Description | Country affiliation: Spain Author Affiliation: Jaramillo Mdel C ( Institute for Bioengineering of Catalonia (IBEC), Cellular Biotechnology, Baldiri Reixac 15-21, 08028 Barcelona, Spain.) |
| Abstract | Dielectrophoresis (DEP) is a powerful tool to manipulate cells and molecules in microfluidic chips. However, few practical applications using DEP exist. An immediate practical application of a carbon-electrode DEP system, in removing PCR inhibitors from a sample, is reported in this work. We use a high throughput carbon-electrode DEP system to trap yeast cells from a natural sample (fermented grape must) and then in situ remove contaminants that interfere with PCR analysis. Retrieval of this enriched and purified yeast population from the DEP system then allows for a significant increase of sensitivity during PCR analysis. Furthermore, the fact that DEP can discriminate between viable and non-viable cells minimizes the number of false positives commonly obtained when using PCR alone. Experimental results provide clear evidence that the carbon-electrode DEP-based sample preparation step can readily and effectively clean environmental samples from natural contaminants and improve PCR sensitivity. |
| ISSN | 09565663 |
| Volume Number | 43 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-05-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Cell Separation Instrumentation Dna-directed Dna Polymerase Isolation & Purification Electrophoresis Environmental Monitoring Polymerase Chain Reaction Saccharomyces Cerevisiae Genetics Equipment Design Equipment Failure Analysis Reproducibility Of Results Sensitivity And Specificity Specimen Handling Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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