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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ooya, Tooru Takeuchi, Toshifumi Murata, Akiko |
| Description | Country affiliation: Japan Author Affiliation: Murata A ( Graduate School of Engineering, Kobe University, 1-1, Kobe 657-8501, Japan.) |
| Abstract | Simple method to immobilize antibody in organic/inorganic hybrid thin films, prepared easily by using liquid phase deposition (LPD), was proposed to enhance the sensitivity, reproducibility and stability of immunosensing chips. The LPD method could embed a monoclonal antibody of C-reactive protein (Anti-CRP), which is mixed with poly(l-lysine) (PL) as an organic binder, on/in titanium oxide thin layer deposited onto a surface plasmon resonance (SPR) sensing chip (F(Anti-CRP-PL)), allowing simple immobilization of anti-CRP capable of CRP detection. As a control, anti-CRP was non-covalently immobilized after preparing the titanium oxide layer containing PL (SIF(Anti-CRP-PL)). Binding experiments of CRP on the prepared sensor chips were performed by SPR measurements. The CRP binding capacity of anti-CRP on F(Anti-CRP-PL) was 54% greater than that of anti-CRP on SIF (Anti-CRP-PL), suggesting that the immobilization method could retain good antigen-binding activity of anti-CRP. Reproducibility of the antigen-antibody interaction was estimated by SPR using 10mM HCl as the regeneration regent. CRP was repeatedly detected, and the detection limit was calculated to be 19.9ngmL(-1) (CV: 8.9%). By using this method, antibodies could be simply immobilized to various kinds of sensing chips for the development of immunosensing systems. |
| ISSN | 09565663 |
| Volume Number | 43 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-05-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Antibodies Chemistry Antigens Analysis Immunoassay Instrumentation Inorganic Chemicals Membranes, Artificial Organic Chemicals Surface Plasmon Resonance Adsorption Immunology Biosensing Techniques Equipment Design Equipment Failure Analysis Journal Article Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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