NDLI: Label-free electrochemical IgE aptasensor based on covalent attachment of aptamer onto multiwalled carbon nanotubes/ionic liquid/chitosan nanocomposite modified electrode.

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Label-free electrochemical IgE aptasensor based on covalent attachment of aptamer onto multiwalled carbon nanotubes/ionic liquid/chitosan nanocomposite modified electrode.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Khezrian, Somayeh Teymourian, Hazhir Hallaj, Rahman Salimi, Abdollah
Description	Country affiliation: Iran Author Affiliation: Khezrian S ( Department of Chemistry, University of Kurdistan, P. O. Box 416, Sanandaj, Iran.)
Abstract	A simple, sensitive and label-free aptamer-based biosensor for the detection of human immunoglobulin E (IgE) is developed using the electrochemical transduction method. A special immobilization interface consisting of multiwalled carbon nanotubes/ionic liquid/chitosan nanocomposite (MWCNTs/IL/Chit) is utilized to improve the conductivity and performance characteristics of the biosensor as well as to increase the loading amount of aptamer DNA sequence. A 5'-amino-terminated aptamer is covalently attached onto MWCNTs/IL/Chit modified glassy carbon (GC) electrode via a linker of glutaraldehyde (GA). Methylene blue (MB) is used as an electrochemical indicator which is intercalated into the aptamer through the specific interaction with its guanine bases. In the absence of IgE, MB bound to the aptamer produces a strong differential pulse voltammetric (DPV) signal. But when IgE exists, the intercalated MB releases from the aptamer, resulting an obviously decreased DPV signal. This phenomenon can be applied for human IgE detection. The peak current of MB linearly decreases with the concentration of IgE over a range of 0.5-30 nM with a detection limit of 37 pM. By using Bovine serum albumin (BSA) and lysozyme, the excellent specificity of this sensing system for the detection of IgE is also demonstrated. Finally, the proposed aptasensor is successfully used to IgE analysis in human serum sample. The obtained result is well agreed with the value obtained by the standard ELISA method. The herein described approach is expected to promote the exploitation of aptamer-based biosensors for protein assays in biochemical and biomedical studies.
ISSN	09565663
Volume Number	43
e-ISSN	18734235
Journal	Biosensors and Bioelectronics
Language	English
Publisher	Elsevier
Publisher Date	2013-05-15
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Aptamers, Nucleotide Chemistry Biosensing Techniques Instrumentation Chitosan Conductometry Electrodes Immunoglobulin E Blood Ionic Liquids Nanotubes, Carbon Equipment Design Equipment Failure Analysis Ultrastructure Staining And Labeling Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry

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