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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Unnikrishnan, Binesh Weng, Cheng-I Lin, Chia-Hua Li, Yu-Jia Chang, Huan-Tsung Huang, Chih-Ching Shen, Yu-Wei |
| Description | Country affiliation: Taiwan Author Affiliation: Weng CI ( Department of Bioscience and Biotechnology, NationalTaiwan Ocean University, 2, Pei-Ning Road, Keelung 20224, Taiwan.); Chang HT ( Department of Chemistry, National Taiwan University, Taipei 10617, Taiwan.); Lin CH ( Key Laboratory of Nano-Bio Interface, Suzhou Key Laboratory for Nanotheranostics, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China.); Shen YW ( Department of Bioscience and Biotechnology, NationalTaiwan Ocean University, 2, Pei-Ning Road, Keelung 20224, Taiwan.); Unnikrishnan B ( Department of Bioscience and Biotechnology, NationalTaiwan Ocean University, 2, Pei-Ning Road, Keelung 20224, Taiwan.); Li YJ ( Department of Bioscience and Biotechnology, NationalTaiwan Ocean University, 2, Pei-Ning Road, Keelung 20224, Taiwan.); Huang CC ( Department of Bioscience and Biotechnology, NationalTaiwan Ocean University, 2, Pei-Ning Road, Keelung 20224, Taiwan) |
| Abstract | In this study, we used a simple one-step dry heating method to synthesize mannose-modified fluorescent carbon quantum dots (Man-CQDs) from solid ammonium citrate and mannose, and successfully applied for labeling Escherichia coli. The highly soluble Man-CQDs had an average particle diameter of 3.1±1.2 nm and exhibited a quantum yield of 9.8% at excitation and emission wavelengths of 365 and 450 nm, respectively. The fluorescent Man-CQDs could selectively bind to the FimH lectin unit in the flagella of the wild-type 1 E. coli K12 strain. We optimized the labeling efficiency of the Man-CQDs by controlling the ratio of ammonium citrate to mannose during their synthesis. The specific binding of the mannose units to E. coli allowed quantitative detection of the bacteria at levels down to 450 colony forming units mL(-1) in lab samples, and facilitate the application of the Man-CQDs for bacterial analyses of real samples (tap water, apple juice, human urine). The synthesis of our Man-CQDs, their labeling, and their use in the detection of bacteria were all simple, inexpensive and efficient processes. |
| ISSN | 09565663 |
| Volume Number | 68 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-06-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Escherichia Coli Isolation & Purification Quantum Dots Chemistry Carbon Citric Acid Humans Mannose Chemical Synthesis Quaternary Ammonium Compounds Staining And Labeling Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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