| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Kim, Do Hyun Kim, Yong Tae Lee, Dohwan Sim, Jeong Eun Heo, Hyun Young Im, Sung Gap Woo, Kwang Man Seo, Tae Seok |
| Description |
Author Affiliation: Kim YT ( Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.); Lee D ( Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.); Heo HY ( Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.); Sim JE ( Forensic Science Division 2, Forensic Science Investigation Department, Supreme Prosecutors' Office, Seoul, Republic of Korea.); Woo KM ( Forensic Science Division 2, Forensic Science Investigation Department, Supreme Prosecutors' Office, Seoul, Republic of Korea.); Kim do H ( Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.); Im SG ( Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.); Seo TS ( Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea. Electronic address: seots@kaist.ac.kr.) |
| Abstract |
A fully integrated slidable and valveless microsystem, which performs solid phase DNA extraction (SPE), micro-polymerase chain reaction (µPCR) and micro-capillary electrophoresis (µCE) coupled with a portable genetic analyser, has been developed for forensic genotyping. The use of a slidable chip, in which a 1 µL-volume of the PCR chamber was patterned at the center, does not necessitate any microvalves and tubing systems for fluidic control. The functional micro-units of SPE, µPCR, and µCE were fabricated on a single glass wafer by conventional photolithography, and the integrated microdevice consists of three layers: from top to bottom, a slidable chip, a channel wafer in which a SPE chamber, a mixing microchannel, and a CE microchannel were fabricated, and a Ti/Pt resistance temperature detector (RTD) wafer. The channel glass wafer and the RTD glass wafer were thermally bonded, and the slidable chip was placed on the designated functional unit. The entire process from the DNA extraction using whole human blood sample to identification of target Y chromosomal short tandem repeat (STR) loci was serially carried out with simply sliding the slidable chamber from one to another functional unit. Monoplex and multiplex detection of amelogenin and mini Y STR loci were successfully analysed on the integrated slidable SPE-µPCR-µCE microdevice by using 1 µL whole human blood within 60 min. The proposed advanced genetic analysis microsystem is capable of point-of-care DNA testing with sample-in-answer-out capability, more importantly, without use of complicated microvalves and microtubing systems for liquid transfer. |
| ISSN |
09565663 |
| Volume Number |
78 |
| e-ISSN |
18734235 |
| Journal |
Biosensors and Bioelectronics |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2016-04-15 |
| Publisher Place |
Great Britain (UK) |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Biosensing Techniques Dna Blood Electrophoresis, Capillary Microsatellite Repeats Genetics Chromosomes, Human, Y Chemistry Genotype Humans Lab-on-a-chip Devices Point-of-care Systems Polymerase Chain Reaction Solid Phase Extraction Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
