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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ang, Shu Hwang Rambeli, Musalman Thevarajah, T. Malathi Alias, Yatimah Binti Khor, Sook Mei |
| Description | Country affiliation: Malaysia Author Affiliation: Ang SH ( Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.); Rambeli M ( Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.); Thevarajah TM ( Department of Pathology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.); Alias YB ( Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.); Khor SM ( Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address: naomikhor@um.edu.my.) |
| Abstract | We describe a gold nanoparticle-based sandwich immunoassay for the dual detection and measurement of hemoglobin A1c (HbA1c) and total hemoglobin in the whole blood (without pretreatment) in a single step for personalized medicine. The optimized antibody-functionalized gold nanoparticles immunoreact simultaneously with HbA1c and total hemoglobin to form a sandwich at distinctive test lines to transduce visible signals. The applicability of this method as a personal management tool was demonstrated by establishing a calibration curve to relate % HbA1c, a useful value for type 2 diabetes management, to the signal ratio of captured HbA1c to all other forms of hemoglobin. The platform showed excellent selectivity (100%) toward HbA1c at distinctive test lines when challenged with HbA0, glycated HbA0 and HbA2. The reproducibility of the measurement was good (6.02%) owing to the dual measurement of HbA1c and total hemoglobin. A blood sample stability test revealed that the quantitative measurement of % HbA1c was consistent and no false-positive results were detected. Also, this method distinguished the blood sample with elevated HbF from the normal samples and the variants. The findings of this study highlight the potential of a lateral flow immunosensor as a simple, inexpensive, consistent, and convenient strategy for the dual measurement of HbA1c and total Hb to provide useful % HbA1c values for better on-site diabetes care. |
| ISSN | 09565663 |
| Volume Number | 78 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-04-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Methods Diabetes Mellitus, Type 2 Blood Hemoglobin A, Glycosylated Isolation & Purification Hemoglobins Gold Chemistry Humans Metal Nanoparticles Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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