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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Xu, Zhiai Zhu, Linling Zhang, Junying Lu, Linlin Wang, Ya Wang, Fengyang Zhang, Wen Feng, Chongchong |
| Description | Author Affiliation: Zhu L ( School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, PR China.); Zhang J ( School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, PR China.); Wang F ( School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, PR China.); Wang Y ( School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, PR China.); Lu L ( School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, PR China.); Feng C ( School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, PR China.); Xu Z ( School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, PR China. Electronic address: zaxu@chem.ecnu.edu.cn.); Zhang W ( School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, PR China. Electronic address: wzhang@chem.ecnu.edu.cn.) |
| Abstract | Amyloid-beta (Aß) oligomers are highly toxic species in the process of Aß aggregation and are regarded as potent therapeutic targets and diagnostic markers for Alzheimer's disease (AD). Herein, a label-free molecular beacon (MB) system integrated with enzyme-free amplification strategy was developed for simple and highly selective assay of Aß oligomers. The MB system was constructed with abasic site (AP site)-containing stem-loop DNA and a fluorescent ligand 2-amino-5,6,7-trimethyl-1,8-naphyridine (ATMND), of which the fluorescence was quenched upon binding to the AP site in DNA stem. Enzyme-free amplification was realized by target-triggered continuous opening of two delicately designed MBs (MB1 and MB2). Target DNA hybridization with MB1 and then MB2 resulted in the release of two ATMND molecules in one binding event. Subsequent target recycling could greatly amplify the detection sensitivity due to the greatly enhanced turn-on emission of ATMND fluorescence. Combining with Aß oligomers aptamers, the strategy was applied to analyze Aß oligomers and the results showed that it could quantify Aß oligomers with high selectivity and monitor the Aß aggregation process. This novel method may be conducive to improve the diagnosis and pathogenic study of Alzheimer's disease. |
| ISSN | 09565663 |
| Volume Number | 78 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-04-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Alzheimer Disease Diagnosis Amyloid Beta-peptides Isolation & Purification Biosensing Techniques Methods Peptide Fragments Enzyme-linked Immunosorbent Assay Humans Ligands Nucleic Acid Hybridization Chemistry Protein Binding Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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