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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Shao, Yong Yin, Zheng-Zhi Zeng, Yan-Bo Zhang, Jian Hu, Rong-Rong Ren, Shi-Bin Li, Lei Liu, Hai-Qing |
| Description | Author Affiliation: Hu RR ( College of Petrochemical Engineering, Changzhou University, Changzhou 213614, PR China); Yin ZZ ( College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing 314001, Zhejiang Province, PR China. Electronic address: yinzhengzhi@mail.zjxu.edu.cn.); Zeng YB ( College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing 314001, Zhejiang Province, PR China.); Zhang J ( College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing 314001, Zhejiang Province, PR China.); Liu HQ ( College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing 314001, Zhejiang Province, PR China.); Shao Y ( College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, PR China.); Ren SB ( School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China.); Li L ( College of Petrochemical Engineering, Changzhou University, Changzhou 213614, PR China) |
| Abstract | New techniques are required for the rapid and sensitive detection of Escherichia coli O157:H7 (E. coli O157:H7), a pathogenic bacterium responsible for serious and sometimes life-threatening diseases in humans. In this study, we developed a highly sensitive and efficient biosensor for the quantitative detection of E. coli O157:H7 by integrating fluorescein-releasable biolabels with a magnetism-separable probe. Hollow silica nanospheres with a diameter of approximately 350 nm were synthesized, enriched with fluorescein, and surface-protected with macromolecule layers of poly (acrylic acid) and poly (dimethyldiallylammonium chloride). These fluorescein-enriched hollow silica nanospheres were characterized using scanning electron microscopy, transmission electron microscopy, and Fourier transform infrared spectroscopy. They were further functionalized as immune labels of E. coli O157:H7 for a sandwich-type immune reaction between this bacterium and magnetic nanoparticles (Fe3O4@SiO2). Next, the E. coli O157:H7 cells were captured, magnetically separated, and quantified based on the fluorescence intensity of the fluorescein released from the biolabels of the fluorescein-enriched hollow silica nanospheres. This analytic process can be completed within 75 min, and the biosensor showed a linear relationship ranging from 4 to 4.0 × 10(8)cfu/mL with a detection limit of 3 cfu/mL. These results show that the developed fluorescent sensor has excellent specificity, and good reproducibility and stability. This study used real spiked samples for detection, indicating that this technique has a wide range of potential applications and may be readily adapted for detecting other pathogens. |
| ISSN | 09565663 |
| Volume Number | 78 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-04-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Methods Escherichia Coli O157 Isolation & Purification Nanospheres Chemistry Silicon Dioxide Pathogenicity Fluorescein Fluorescence Humans Limit Of Detection Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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