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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Che, Yu-Jui Chao, Chih-Yu Wu, Jiunn-Jong Wang, Chih-Hung Kao, Cheng-Yen Lee, Gwo-Bin |
| Description | Country affiliation: Taiwan Author Affiliation: Chao CY ( Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu, Taiwan.); Wang CH ( Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan.); Che YJ ( Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan.); Kao CY ( Department of Medical Laboratory Science and Biotechnology, National Cheng Kung University, Tainan, Taiwan.); Wu JJ ( Department of Medical Laboratory Science and Biotechnology, National Cheng Kung University, Tainan, Taiwan); Lee GB ( Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu, Taiwan) |
| Abstract | Helicobacter pylori (H. pylori) is a species of bacteria that can colonize the human stomach mucosa. It is closely associated with gastric diseases such as ulcer and inflammation. Recently, some H. pylori strains were found to express resistance to a family of antibiotics known as quinolones due to single-point mutations. Although traditional polymerase chain reaction (PCR) and molecular diagnostic-based approaches can be used to determine the presence and abundance of antibiotic-resistant H. pylori strains, such processes are relatively expensive, labor-intensive, and require bulky and costly equipment. This study therefore reports an advanced diagnostic assay performed on an integrated microfluidic system for rapid detection of antibiotic resistance in H. pylori. The assay features three components: (1) nucleic acid extraction by specific probe-conjugated magnetic beads, (2) amplification of the target deoxyribonucleic acid (DNA) fragments by using single-nucleotide-polymorphism polymerase chain reaction (SNP-PCR), and (3) optical detection of the PCR products. The device integrates several microfluidic components including micro-pumps, normally-closed micro-valves, and reaction chambers such that the entire diagnostic assay can be automatically executed on a single microfluidic system within one hour with detection limits of 10(0), 10(2), and 10(2) bacterial cells for H. pylori detection and two different SNP sites strains. Three PCR-based assays for determining presence of H. pylori infection and two DNA single-point mutation assays aimed at determining whether the infected strains were resistant to quinolone can be performed simultaneously on a single chip, suggesting that this microfluidic system could be a promising tool for rapid diagnosis of the presence of antibiotic-resistant H. pylori strains. |
| ISSN | 09565663 |
| Volume Number | 78 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-04-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Dna, Bacterial Genetics Drug Resistance, Microbial Helicobacter Infections Microbiology Helicobacter Pylori Drug Effects Isolation & Purification Drug Therapy Humans Microfluidics Methods Polymorphism, Single Nucleotide Quinolones Therapeutic Use Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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