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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zhao, Shuang Liu, Bo Qin, Yan Ren, Qunxiang Sun, Yingying |
| Description | Author Affiliation: Sun Y ( Department of Chemistry, Shenyang Medical College, Shenyang 110034, China. Electronic address: syyxiluda@hotmail.com.); Ren Q ( Department of Chemistry, Shenyang Medical College, Shenyang 110034, China.); Liu B ( Department of Chemistry, Shenyang Medical College, Shenyang 110034, China.); Qin Y ( Department of Chemistry, Shenyang Medical College, Shenyang 110034, China.); Zhao S ( College of Sciences, Northeastern University, Shenyang 110004, China.) |
| Abstract | An isothermal, enzyme-free and sensitive electrochemical DNA sensor was developed for the detection of the FLT3 gene in acute myeloid leukemia (AML). First, aminated multi-walled carbon nanotubes (AMWNTs) and gold nanoparticles (AuNPs) were alternately self-assembled on a gold electrode using a layer-by-layer strategy. Then, the hairpin DNA probe 1 (H1), with a thiol group at the 3' end and a ferrocenyl moiety (Fc) at the 5' end, was immobilized on the AMWNTs/AuNPs multilayer films through Au-S bonding. When the target DNA (TD) appeared, it hybridized with and opened the hairpin structure of H1, and Fc was forced away from the electrode surface, leading to a significant decrease in the current peak of square wave voltammetry. Subsequently, the hairpin DNA probe 2 (H2) bound to H1, freeing the TD to trigger another reaction cycle. The combination of this target-catalyzed hairpin assembly and the LBL assembly of nanomaterials achieved a detection limit of 0.1 pM with a wide linear range of 0.1-1000 pM. The sensor discriminated between mismatched DNA and the target DNA with high selectivity. This dual signal amplification strategy is relatively simple and inexpensive because it does not need any enzymes or sophisticated equipment and successfully assayed the FLT3 gene from real samples. |
| ISSN | 09565663 |
| Volume Number | 78 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-04-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Methods Leukemia, Myeloid, Acute Diagnosis Nanotubes, Carbon Chemistry Fms-like Tyrosine Kinase 3 Isolation & Purification Electrochemical Techniques Gold Humans Genetics Limit Of Detection Metal Nanoparticles Rna, Small Interfering Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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