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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Blom, Marko Adojutelegan, Adewole Lous, Erik-Jan Stuyver, Lieven J. Frederix, Filip Brivio, Monica Groenhuis, Roelf Van Dorst, Bieke Van Der Sar, Elfried Reuvekamp, Simon Tanzi, Simone |
| Description | Author Affiliation: Van Dorst B ( Janssen Diagnostics BVBA, Beerse, Belgium. Electronic address: bvandors@its.jnj.com.); Brivio M ( Micronit Microfluidics, Twente, The Netherlands.); Van Der Sar E ( NXP Semiconductors, Nijmegen, The Netherlands.); Blom M ( Micronit Microfluidics, Twente, The Netherlands.); Reuvekamp S ( Micronit Microfluidics, Twente, The Netherlands.); Tanzi S ( Micronit Microfluidics, Twente, The Netherlands.); Groenhuis R ( NXP Semiconductors, Nijmegen, The Netherlands.); Adojutelegan A ( NXP Semiconductors, Nijmegen, The Netherlands.); Lous EJ ( NXP Semiconductors, Nijmegen, The Netherlands.); Frederix F ( NXP Semiconductors, Leuven, Belgium.); Stuyver LJ ( Janssen Diagnostics BVBA, Beerse, Belgium.) |
| Abstract | In this manuscript, a microfluidic detection module, which allows a sensitive readout of biological assays in point-of-care (POC) tests, is presented. The proposed detection module consists of a microfluidic flow cell with an integrated Complementary Metal-Oxide-Semiconductor (CMOS)-based single photon counting optical sensor. Due to the integrated sensor-based readout, the detection module could be implemented as the core technology in stand-alone POC tests, for use in mobile or rural settings. The performance of the detection module was demonstrated in three assays: a peptide, a protein and an antibody detection assay. The antibody detection assay with readout in the detection module proved to be 7-fold more sensitive that the traditional colorimetric plate-based ELISA. The protein and peptide assay showed a lower limit of detection (LLOD) of 200 fM and 460 fM respectively. Results demonstrate that the sensitivity of the immunoassays is comparable with lab-based immunoassays and at least equal or better than current mainstream POC devices. This sensitive readout holds the potential to develop POC tests, which are able to detect low concentrations of biomarkers. This will broaden the diagnostic capabilities at the clinician's office and at patient's home, where currently only the less sensitive lateral flow and dipstick POC tests are implemented. |
| ISSN | 09565663 |
| Volume Number | 78 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-04-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Antibodies Isolation & Purification Biosensing Techniques Microfluidic Analytical Techniques Methods Peptides Proteins Chemistry Biological Assay Colorimetry Humans Metals Instrumentation Oxides Point-of-care Testing Semiconductors Journal Article Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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