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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Phua, Szechao Li, Zhi Yan, Jinyong Li, Aitao Xu, Yi Ngo, Thao P. N. |
| Description | Country affiliation: Singapore Author Affiliation: Yan J ( Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117576, Singapore.) |
| Abstract | A novel concept and efficient method for producing biodiesel (FAME) from grease (15-40wt% free fatty acid, FFA) were developed by using tandem lipases for one-pot esterification of FFA and transesterification of triglyceride with methanol in a solvent-free system. Combining immobilized Candida antarctica lipase B (CALB) (Novozyme 435) favoring the esterification and immobilized Thermomyces lanuginosus lipase (TLL) (Lipozyme TLIM) preferring the transesterification at 2:8 (wt/wt) gave FAME in 80% yield, being better than that with Novozyme 435 or Lipozyme TLIM. Recombinant Escherichia coli (Calb/Tll) co-expressing CALB and TLL was engineered as a more efficient tandem-lipases system. Using wet or dry cells (4wt%) gave FAME in 87% or 95% yield, which is much better than that with E. coli cells expressing either CALB or TLL alone. Cells of E. coli (Calb/Tll) were recycled for five times and retained 75% productivity, thus being practical for producing biodiesel from grease. |
| ISSN | 09608524 |
| Volume Number | 123 |
| e-ISSN | 18732976 |
| Journal | Bioresource Technology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-11-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biofuels Analysis Biotechnology Methods Lipase Metabolism Oils Waste Products Ascomycota Enzymology Biotransformation Candida Electrophoresis, Polyacrylamide Gel Enzymes, Immobilized Escherichia Coli Cytology Esterification Esters Fatty Acids Methanol Recombination, Genetic Genetics Recycling Time Factors Journal Article Research Support, Non-u.s. Gov't Discipline Bioresource |
| Content Type | Text |
| Resource Type | Article |
| Subject | Waste Management and Disposal Medicine Environmental Engineering Renewable Energy, Sustainability and the Environment Bioengineering |
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