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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Jhamb, Kamna Sahoo, Debendra K. |
| Description | Country affiliation: India Author Affiliation: Jhamb K ( CSIR - Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India.) |
| Abstract | In this study, effects of temperature, inducer concentration, time of induction and co-expression of molecular chaperones (GroEL-GroES and DnaKJE), on cell growth and solubilization of model protein, xylanases, were investigated. The yield of soluble xylanases increased with decreasing cultivation temperature and inducer level. In addition, co-expression of DnaKJE chaperone resulted in increased soluble xylanases though the time of induction of chaperone and target protein had a bearing on this yield. A combination of chaperone co-expression and partial induction resulted in â ¼40% (in DnaKJE) and 33% (in GroEL-GroES) of total xylanase yield in soluble fraction. However, the conditions for maximum yield of soluble r-XynB and maximum % soluble expression of r-XynB were different. Higher expression of soluble xylanases in a scalable semi-synthetic medium showed potential of the process for soluble enzyme production. |
| ISSN | 09608524 |
| Volume Number | 123 |
| e-ISSN | 18732976 |
| Journal | Bioresource Technology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-11-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biotechnology Methods Endo-1,4-beta Xylanases Biosynthesis Escherichia Coli Growth & Development Metabolism Molecular Chaperones Recombinant Proteins Cytology Enzymology Isopropyl Thiogalactoside Pharmacology Solubility Temperature Time Factors Journal Article Research Support, Non-u.s. Gov't Discipline Bioresource |
| Content Type | Text |
| Resource Type | Article |
| Subject | Waste Management and Disposal Medicine Environmental Engineering Renewable Energy, Sustainability and the Environment Bioengineering |
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