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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Paek, Kee-Yoeup Jin, You-Xun Cui, Xi-Hua Yim, Yong-Hyeon |
| Description | Author Affiliation: Jin YX ( Center for Analytical Chemistry, Division of Metrology for Quality of Life, Korea.) |
| Abstract | An efficient enrichment method using immobilized metal affinity chromatography (IMAC) was developed for selective extraction of bioactive sphingoid base-1-phosphates (SB1Ps) from adventitious roots of Hypericum perforatum cultured in bioreactor. The phosphate-selective IMAC enrichment coupled with LC-MS/MS enabled sensitive analysis of low-abundance SB1Ps present in the root biomass, which would not be feasible otherwise due to severe interferences from complex biological matrices. The time-dependent growth rate and production of SB1Ps from adventitious roots were investigated. The level of phytosphingosine-1-phosphate, which was found to be the major SB1Ps, reached a maximum amount of 635.6pmolpergram of dry weight after 3weeks of culture and decreased between 3 and 5weeks of culture subsequently. On the other hand, sphingosine-1-phosphate and sphinganine-1-phosphate were present at levels of 18.91 and 73.15pmolpergram of dry weight, respectively, after a week of culture and their level decreased thereafter. |
| ISSN | 09608524 |
| Volume Number | 123 |
| e-ISSN | 18732976 |
| Journal | Bioresource Technology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-11-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Bioreactors Biotechnology Instrumentation Methods Hypericum Metabolism Lysophospholipids Biosynthesis Sphingosine Analogs & Derivatives Chloroform Chemistry Chromatography, Affinity Chromatography, High Pressure Liquid Growth & Development Mass Spectrometry Metals Methanol Plant Roots Solvents Time Factors Journal Article Research Support, Non-u.s. Gov't Discipline Bioresource |
| Content Type | Text |
| Resource Type | Article |
| Subject | Waste Management and Disposal Medicine Environmental Engineering Renewable Energy, Sustainability and the Environment Bioengineering |
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