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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Romdhane, Ines Belhaj-Ben Romdhane, Zamen Ben Bouzid, Maha Gargouri, Ali Belghith, Hafedh |
| Description | Author Affiliation: Romdhane IB ( Laboratory of Biomass Valorization and Proteins Production in Eukaryotes, Center of Biotechnology of Sfax, University of Sfax, PB 1177, 3038, Sfax, Tunisia, inesbelhaj@yahoo.fr.) |
| Abstract | Waste frying oil, which not only harms people's health but also causes environmental pollution, can be a good alternative to partially substitute petroleum diesel through transesterification reaction. This oil contained 8.8 % of free fatty acids, which cause a problem in a base-catalyzed process. In this study, synthesis of biodiesel was efficiently catalyzed by the covalently immobilized Talaromyces thermophilus lipase and allowed bioconversion yield up to 92 % after 24 h of reaction time. The optimal molar ratio was four to six parts of methanol to one part of oil with a biocatalyst loaded of 25 wt.% of oil. Further, experiments revealed that T. thermophilus lipase, immobilized by a multipoint covalent liaison onto activated chitosan via a short spacer (glutaraldehyde), was sufficiently tolerant to methanol. In fact, using the stepwise addition of methanol, no significant difference was observed from the one-step whole addition at the start of reaction. The batch biodiesel synthesis was performed in a fixed bed reactor with a lipase loaded of 10 g. The bioconversion yield of 98 % was attained after a 5-h reaction time. The bioreactor was operated successfully for almost 150 h without any changes in the initial conversion yield. Most of the chemical and physical properties of the produced biodiesel meet the European and USA standard specifications of biodiesel fuels. |
| File Format | HTM / HTML |
| ISSN | 02732289 |
| Issue Number | 8 |
| Volume Number | 171 |
| e-ISSN | 15590291 |
| Journal | Applied Biochemistry and Biotechnology |
| Language | English |
| Publisher | Springer |
| Publisher Date | 2013-12-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry Discipline Biotechnology Bioreactors Chitosan Chemistry Lipase Waste Products Biofuels Enzymes, Immobilized Esterification Humans Methanol Plant Oils Talaromyces Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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