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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Hsu, Fu-Yuan Chen, Yo-Chia Cheng, Hsueh-Ling Chiang, Yu-Chuan Tsai, Li-Chu |
| Description | Author Affiliation: Chen YC ( Department of Biological Science and Technology, National Pingtung University of Science and Technology, 1, Shuehfu Rd., Neipu, Pingtung 91201, Taiwan, ROC.) |
| Abstract | Rumen fungi are a rich source of enzymes degrading lignocelluloses. XynR8 is a glycosyl hydrolase family 11 xylanase previously cloned from unpurified rumen fungal cultures. Phylogenetic analysis suggested that xynR8 was obtained from a Neocallimastix species. Recombinant XynR8 expressed in Escherichia coli was highly active and stable between pH 3.0 and 11.0, and displayed a V(max) of 66,672µmolmin(-1)mg(-1), a k(cat) of 38,975s(-1), and a K(m) of 11.20mg/mL towards soluble oat spelt xylan. Based on molecular modeling, residues N41 and N58, important in stabilizing two loops and the structure of XynR8, were mutated to D. Both mutant enzymes showed higher tolerance to pH 2.0. The V(max), k(cat) and K(m) of the N41D and N58D mutant enzymes were 79,645µmolmin(-1)mg(-1), 46,493s(-1), 29.29mg/mL, and 96,689µmolmin(-1)mg(-1), 56,503s(-1), and 21.24mg/mL, respectively. Thus, they are good candidates for application, including biofuel production. |
| ISSN | 09608524 |
| Volume Number | 123 |
| e-ISSN | 18732976 |
| Journal | Bioresource Technology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-11-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Endo-1,4-beta Xylanases Chemistry Metabolism Models, Molecular Neocallimastix Enzymology Rumen Microbiology Amino Acid Sequence Animals Cells, Cultured Chromatography, Thin Layer Genetics Isolation & Purification Enzyme Stability Fungal Proteins Hydrogen-ion Concentration Hydrolysis Kinetics Molecular Sequence Data Mutagenesis, Site-directed Mutant Proteins Phylogeny Recombinant Proteins Substrate Specificity Temperature Journal Article Research Support, Non-u.s. Gov't Discipline Bioresource |
| Content Type | Text |
| Resource Type | Article |
| Subject | Waste Management and Disposal Medicine Environmental Engineering Renewable Energy, Sustainability and the Environment Bioengineering |
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