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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Huang, Ting Yen Lu, Wen Chang Chu, I. Ming |
| Description | Author Affiliation: Huang TY ( Chemical Engineering Department, National Tsing Hua University, Hsinchu, Taiwan, ROC.) |
| Abstract | During the fermentation process, dissolved oxygen values and carbon-to-nitrogen ratios are critical factors influencing DHA productivity. This study employed an intermittent oxygen feeding method to maintain a 50% dissolved oxygen level and produced a dissolved oxygen fluctuation environment to facilitate both Aurantiochytrium limacinum SR21 growth and lipid accumulation. Study results indicated that at a 1.25 C:N ratio and medium composition of 100gL(-1) glycerol, 40gL(-1) yeast extract, and 40gL(-1) peptone, A. limacinum SR21 achieved biomass at 61.76gL(-1), lipid content at 65.2%, DHA concentration at 20.3gL(-1), and DHA productivity at 122.62mgL(-1)h(-1), this result were better than most similar researches. Dissolved oxygen fluctuation environment also altered the fatty acid composition of A. limacinum SR21. In the late period of the fermentation process, C16:0 fatty acid ratios decreased significantly to below 5%, and C22:6 fatty acid ratios increased to 70%. |
| ISSN | 09608524 |
| Volume Number | 123 |
| e-ISSN | 18732976 |
| Journal | Bioresource Technology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-11-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biotechnology Methods Docosahexaenoic Acids Biosynthesis Fatty Acids Chemistry Metabolism Fermentation Stramenopiles Batch Cell Culture Techniques Biomass Carbon Pharmacology Drug Effects Glycerol Growth & Development Journal Article Research Support, Non-u.s. Gov't Discipline Bioresource |
| Content Type | Text |
| Resource Type | Article |
| Subject | Waste Management and Disposal Medicine Environmental Engineering Renewable Energy, Sustainability and the Environment Bioengineering |
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