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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Paek, Kee-Yoeup Jin, You-Xun Murthy, Hosakatte Niranjana Kim, Ji-Yeong Cui, Xi-Hua Yim, Yong-Hyeon |
| Description | Author Affiliation: Cui XH ( Research Center for Development of Advanced Horticultural Technology, Chungbuk National University, Chungju 361-763, Republic of Korea.) |
| Abstract | The effects of inoculum density, aeration volume and culture period on accumulation of biomass and secondary metabolites in adventitious roots of Hypericum perforatum in balloon type airlift bioreactors (3 l capacity) were investigated. The greatest increment of biomass as well as metabolite content occurred at an inoculum density of 3 g l(-1) and an aeration volume of 0.1 vvm. After 6 weeks of culture, an approximately 50-fold increase in biomass was recorded containing 60.11 mg g(-1) dry weight (DW) of phenolics, 42.7 mg g(-1) DW of flavonoids and 0.80 mg g(-1) DW of chlorogenic acid. Liquid chromatography-mass spectroscopy/mass spectroscopy demonstrated that the presence of quercetin and hyperoside in adventitious roots at a level of 1.33 and 14.01 µg g(-1) DW, respectively after 6 weeks of culture. The results suggest scale-up of adventitious root culture of H. perforatum for the production of chlorogenic acid, quercetin and hyperoside is feasible. |
| ISSN | 09608524 |
| Issue Number | 21 |
| Volume Number | 102 |
| e-ISSN | 18732976 |
| Journal | Bioresource Technology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2011-11-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biomass Bioreactors Biotechnology Instrumentation Methods Hypericum Growth & Development Metabolism Plant Roots Antioxidants Biphenyl Compounds Chromatography, Liquid Flavonoids Hydrogen Peroxide Hydrogen-ion Concentration Kinetics Malondialdehyde Phenols Picrates Quercetin Analogs & Derivatives Chemistry Isolation & Purification Spectrometry, Mass, Electrospray Ionization Journal Article Discipline Bioresource |
| Content Type | Text |
| Resource Type | Article |
| Subject | Waste Management and Disposal Medicine Environmental Engineering Renewable Energy, Sustainability and the Environment Bioengineering |
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