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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Hua, Chengwei Li, Lite Jiang, Zhengqiang Yang, Shaoqing Zhang, Min |
| Description | Country affiliation: China Author Affiliation: Zhang M ( Department of Biotechnology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.) |
| Abstract | A cDNA library of Paecilomyces thermophila was constructed, and the gene encoding xylanase (designated Pt xynA) was isolated from the library. Pt xynA consisted of 681 bp, and the translated protein encoded 226 amino acids. This is the first functional gene cloned from P. thermophila. The gene was successfully expressed in Escherichia coli BL21 and the recombinant xylanase (XynA) was purified to homogeneity by Ni-NTA and Sephadex G50. XynA showed an optimum activity at 75 degrees C and pH 7.0. Its residual activity was more than 60% after being treated at 85 degrees C for 30 min. K(m) values of XynA for birchwood xylan, beechwood xylan and oat-spelt xylan were 4.4, 3.6 and 9.7 mg ml(-1), respectively. The enzyme has an endohydrolytic mode of action and can hydrolyse xylotriose to xylobiose through transglycosylation. These results indicate the XynA is a thermostable enzyme and has great potential in various industries. |
| ISSN | 09608524 |
| Issue Number | 2 |
| Volume Number | 101 |
| e-ISSN | 18732976 |
| Journal | Bioresource Technology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-01-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Endo-1,4-beta Xylanases Genetics Paecilomyces Amino Acid Sequence Base Sequence Cloning, Molecular Dna Primers Dna, Fungal Electrophoresis, Polyacrylamide Gel Escherichia Coli Genes, Fungal Hydrogen-ion Concentration Molecular Sequence Data Enzymology Recombinant Proteins Temperature Journal Article Research Support, Non-u.s. Gov't Discipline Bioresource |
| Content Type | Text |
| Resource Type | Article |
| Subject | Waste Management and Disposal Medicine Environmental Engineering Renewable Energy, Sustainability and the Environment Bioengineering |
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