NDLI: Formation of pilin in Pseudomonas aeruginosa requires the alternative sigma factor (RpoN) of RNA polymerase.

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Formation of pilin in Pseudomonas aeruginosa requires the alternative sigma factor (RpoN) of RNA polymerase.

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Formation of pilin in Pseudomonas aeruginosa requires the alternative sigma factor (RpoN) of RNA polymerase.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Ishimoto, K. S. Lory, S.
Description	Author Affiliation: Ishimoto KS ( Department of Microbiology, School of Medicine, University of Washington, Seattle 98195.);
Abstract	The promoter region of the Pseudomonas aeruginosa pilin gene has a high degree of similarity to the nitrogen-regulated promoters of enteric bacteria. These promoters are recognized by the alternative sigma factor of RNA polymerase, termed RpoN (NtrA or GlnF). This observation suggested that the P. aeruginosa pilin gene may be transcribed by the RpoN-containing RNA polymerase. We, therefore, cloned the RpoN gene from P. aeruginosa into Escherichia coli (where it formed a functional product) and used that cloned gene to construct a mutant of P. aeruginosa that was insertionally inactivated in its RpoN gene. This mutant failed to synthesize pilin, indicating that the RpoN sigma factor is required for transcription of the pilin gene.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	6
Volume Number	86
Language	English
Publisher	National Academy of Sciences
Publisher Date	1989-04-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Bacterial Outer Membrane Proteins Genetics DNA-Directed RNA Polymerases Pseudomonas Aeruginosa Sigma Factor Transcription Factors Biosynthesis Cloning, Molecular Escherichia Coli Fimbriae Proteins Fimbriae, Bacterial Gene Expression Regulation Mutation Nucleic Acid Hybridization Plasmids Promoter Regions, Genetic Transcription, Genetic Transformation, Bacterial Beta-Galactosidase Metabolism Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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