NDLI: Discovery of sulfated metabolites in mycobacteria with a genetic and mass spectrometric approach.

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Discovery of sulfated metabolites in mycobacteria with a genetic and mass spectrometric approach.

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Discovery of sulfated metabolites in mycobacteria with a genetic and mass spectrometric approach.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Williams, Spencer J. Riley, Lee W. Senaratne, Ryan H. Mougous, Joseph D. Leavell, Michael D. Leigh, Clifton D. Leary, Julie A. Bertozzi, Carolyn R.
Description	Author Affiliation: Mougous JD ( Howard Hughes Medical Institute, Department of Molecular and Cell Biology, Department of Chemistry, and School of Public Health, University of California, Berkeley, CA 94720, USA.);
Abstract	The study of the metabolome presents numerous challenges, first among them being the cataloging of its constituents. A step in this direction will be the development of tools to identify metabolites that share common structural features. The importance of sulfated molecules in cell-cell communication motivated us to develop a rapid two-step method for identifying these metabolites in microorganisms, particularly in pathogenic mycobacteria. Sulfurcontaining molecules were initially identified by mass spectral analysis of cell extracts from bacteria labeled metabolically with a stable sulfur isotope (34SO 4 2-). To differentiate sulfated from reduced-sulfur-containing molecules, we employed a mutant lacking the reductive branch of the sulfate assimilation pathway. In these sulfur auxotrophs, heavy sulfate is channeled exclusively into sulfated metabolites. The method was applied to the discovery of several new sulfated molecules in Mycobacterium tuberculosis and Mycobacterium smegmatis. Because a sulfur auxotrophic strain is the only requirement of the approach, many microorganisms can be studied in this manner. Such genetic engineering in combination with stable isotopic labeling can be applied to various metabolic pathways and their products.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	26
Volume Number	99
Language	English
Publisher	National Academy of Sciences
Publisher Date	2002-12-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Disaccharides Metabolism Mycobacterium Smegmatis Mycobacterium Tuberculosis Pyrazoles Sulfhydryl Compounds Sulfoglycosphingolipids Cysteine Genetic Engineering Glycopeptides Inositol Mass Spectrometry Sulfates Sulfur Isotopes Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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