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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Yan, Fenggen Wang, Mei Li, Jiaming Cheng, Hui Su, Jingjing Wang, Xiaoshan Wu, Haiyun Xia, Lunzhu Li, Xiaoxiang Chang, Hebron C. Li, Qinglin |
| Description | Country affiliation: China Author Affiliation: Yan F ( Key Laboratory of Xin'an Medicine, Ministry of Education, Anhui Province Key Laboratory of R&D of Chinese Medicine, Anhui University of Traditional Chinese Medicine, Hefei 230038, China.) |
| Abstract | Gambogenic acid, identified from Gamboge, is responsible for anti-tumor effects, and has been shown to be a potential molecule against human cancers. In this study, the molecular mechanism of gambogenic acid-induced apoptosis in HepG2 cells was investigated. Gambogenic acid significantly inhibited cell proliferation and induced apoptosis. Acridine orange/ethidium bromide (AO/EB) staining was used to observe apoptosis, and then confirmed by transmission electron microscopy. Gambogenic acid induced apoptosis and morphological changes in mitochondria, and intracellular reactive oxygen species (ROS) and mitochondrial membrane permeabilization (MMP) in mitochondrial apoptosis pathway were also examined. Results showed that the levels of phospho-p38 and its downstream phospho-Erk1/2 of HepG2 cells increased in time- and concentration-dependent manners after gambogenic acid treatments. Additionally, gambogenic acid increased expression ratio of Bcl-2/Bax in mRNA levels, Western blotting analysis also further confirmed the reduced level of Bcl-2 and increase the expression level of Bax in HepG2 cells. These results indicated that gambogenic acid induced mitochondrial oxidative stress and activated caspases through a caspase-3 and caspase-9-dependent apoptosis pathway. Moreover, gambogenic acid mediated apoptosis and was involved in the phospho-Erk1/2 and phospho-p38 MAPK proteins expression changes in HepG2 cells. |
| File Format | HTM / HTML |
| ISSN | 13826689 |
| Issue Number | 2 |
| Volume Number | 33 |
| Journal | Environmental Toxicology and Pharmacology |
| e-ISSN | 18727077 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-03-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Environmental Health Discipline Pharmacology Antineoplastic Agents, Phytogenic Pharmacology Apoptosis Drug Effects Carcinoma, Hepatocellular Enzymology Liver Neoplasms Mitochondria Mitogen-activated Protein Kinase 1 Metabolism Mitogen-activated Protein Kinase 3 Terpenes Xanthones P38 Mitogen-activated Protein Kinases Blotting, Western Genetics Pathology Caspase 3 Caspase 9 Cell Proliferation Cell Shape Cell Survival Dose-response Relationship, Drug Hep G2 Cells Humans Membrane Potential, Mitochondrial Microscopy, Electron, Transmission Oxidative Stress Phosphorylation Proto-oncogene Proteins C-bcl-2 Rna, Messenger Reactive Oxygen Species Signal Transduction Time Factors Xanthenes Bcl-2-associated X Protein Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Health, Toxicology and Mutagenesis Medicine Toxicology Pharmacology |
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