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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Qiu, Jing Zhu, Guoying Chen, Xiao Shao, Chunlin Gu, Shuzhu |
| Description | Country affiliation: China Author Affiliation: Qiu J ( Department of Environmental Epidemiology and Bone Toxicology, Institute of Radiation Medicine, Fudan University, China.) |
| Abstract | The combined effects of γ-irradiation and cadmium (Cd) exposures on osteoblasts were observed in the present study. Osteoblasts were exposed to γ-irradiation (0.5 Gy) and Cd (0-0.5 µmol/L). Cell viability, alkaline phosphatase (ALP) activity, mineralization ability, cell apoptosis and genes expression of ALP, osteocalcin (OC) and caspase 3 were observed. Low concentrations of Cd exposure had no obvious influence on cell viability, ALP activity and apoptosis. However, low levels of Cd exposure combined with γ-irradiation induced more toxic effects on osteoblasts than those treated with Cd or irradiation alone. High concentrations of Cd combined with irradiation exposure induced more significant inhibition in cell viability, ALP activity and mineralization ability than those exposed to Cd or irradiation alone. Meanwhile, OC and ALP mRNA expression of cells treated with Cd combined with irradiation were down-regulated more significantly than those treated with Cd or irradiation alone. Cd combined with γ-irradiation could obviously enhance osteoblast apoptosis and up-regulated caspase 3 mRNA expression compared with those treated with Cd or irradiation alone. This study indicated that ionizing irradiation can enhance Cd toxic effects on osteoblast viability and differentiation and apoptosis may play an important role in this progress. |
| File Format | HTM / HTML |
| ISSN | 13826689 |
| Issue Number | 2 |
| Volume Number | 33 |
| e-ISSN | 18727077 |
| Journal | Environmental Toxicology and Pharmacology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-03-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Environmental Health Discipline Pharmacology Cadmium Chloride Toxicity Environmental Pollutants Gamma Rays Osteoblasts Drug Effects Radiation Effects Alkaline Phosphatase Genetics Metabolism Animals Apoptosis Calcification, Physiologic Caspase 3 Cell Differentiation Cell Proliferation Cell Shape Cell Survival Cells, Cultured Dose-response Relationship, Drug Gene Expression Regulation Osteocalcin Rna, Messenger Rats Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Health, Toxicology and Mutagenesis Medicine Toxicology Pharmacology |
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