| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Kaisarevic, Sonja Dakic, Vanja Hrubik, Jelena Glisic, Branka Lübcke-von Varel, Urte Pogrmic-Majkic, Kristina Fa, Svetlana Teodorovic, Ivana Brack, Werner Kovacevic, Radmila |
| Description |
Author Affiliation: Kaisarevic S ( University of Novi Sad Faculty of Sciences, Department of Biology and Ecology, Trg D. Obradovica 2, 21000 Novi Sad, Serbia. Electronic address: sonja.kaisarevic@dbe.uns.ac.rs.); Dakic V ( University of Novi Sad Faculty of Sciences, Department of Biology and Ecology, Trg D. Obradovica 2, 21000 Novi Sad, Serbia.); Hrubik J ( University of Novi Sad Faculty of Sciences, Department of Biology and Ecology, Trg D. Obradovica 2, 21000 Novi Sad, Serbia.); Glisic B ( University of Novi Sad Faculty of Sciences, Department of Biology and Ecology, Trg D. Obradovica 2, 21000 Novi Sad, Serbia.); Lübcke-von Varel U ( Helmholtz Centre for Environmental Research - UFZ, Department of Effect-Directed Analysis, Permoserstr. 15, 04318 Leipzig, Germany.); Pogrmic-Majkic K ( University of Novi Sad Faculty of Sciences, Department of Biology and Ecology, Trg D. Obradovica 2, 21000 Novi Sad, Serbia.); Fa S ( University of Novi Sad Faculty of Sciences, Department of Biology and Ecology, Trg D. Obradovica 2, 21000 Novi Sad, Serbia.); Teodorovic I ( University of Novi Sad Faculty of Sciences, Department of Biology and Ecology, Trg D. Obradovica 2, 21000 Novi Sad, Serbia.); Brack W ( Helmholtz Centre for Environmental Research - UFZ, Department of Effect-Directed Analysis, Permoserstr. 15, 04318 Leipzig, Germany.); Kovacevic R ( University of Novi Sad Faculty of Sciences, Department of Biology and Ecology, Trg D. Obradovica 2, 21000 Novi Sad, Serbia.) |
| Abstract |
Rat hepatoma cells H4IIE were treated by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and polycyclic aromatic hydrocarbons (PAHs) (dibenz(a,h)anthracene, benzo(a)pyrene, benz(a)anthracene, chrysene), low-concentration mixtures of PAHs and TCDD, and environmental mixtures contaminated by PAHs and their derivatives. Expression of the gene battery comprising cytochrome P450 Cyp1a1, Cyp1a2, Cyp1b1, and glutathione-s-transferase Gsta2 and Gstp was investigated using quantitative real time polymerase chain reaction (qRT-PCR) analysis. The results revealed that TCDD induce Cyp1a1>Cyp1a2>Cyp1b1, while PAHs and PAH-containing environmental mixtures induce Cyp1a2>Cyp1a1>Cyp1b1 gene expression pattern. While low-concentration mixtures elicited a more pronounced response in comparison to single treatments, the typical gene expression patterns were not observed. In all samples, Gsta2 was predominantly expressed relative to Gstp. These findings indicate that differential Cyp1a1 and Cyp1a2 expression in the H4IIE cells might be used for detection of PAHs in highly contaminated environmental mixtures, but not in low-concentration mixtures of these compounds. |
| File Format |
HTM / HTML |
| ISSN |
13826689 |
| Issue Number |
1 |
| Volume Number |
39 |
| e-ISSN |
18727077 |
| Journal |
Environmental Toxicology and Pharmacology |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2015-01-01 |
| Publisher Place |
Netherlands |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Discipline Environmental Health Discipline Pharmacology Cytochrome P-450 Cyp1a1 Genetics Cytochromes Gene Expression Regulation, Enzymologic Drug Effects Gene Expression Regulation, Neoplastic Toxicity Polycyclic Hydrocarbons, Aromatic Animals Carcinoma, Hepatocellular Cell Line, Tumor Cytochrome P-450 Cyp1a2 Cytochrome P-450 Cyp1b1 Glutathione S-transferase Pi Glutathione Transferase Isoenzymes Liver Neoplasms Rats Journal Article Research Support, Non-u.s. Gov't |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Health, Toxicology and Mutagenesis Medicine Toxicology Pharmacology |
