NDLI: Particle size modeling and morphology study of chitosan/gelatin/nanohydroxyapatite nanocomposite microspheres for bone tissue engineering.

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Particle size modeling and morphology study of chitosan/gelatin/nanohydroxyapatite nanocomposite microspheres for bone tissue engineering.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Bagheri-Khoulenjani, Shadab Mirzadeh, Hamid Etrati-Khosroshahi, Mohammad Shokrgozar, Mohammad Ali
Description	Country affiliation: Iran Author Affiliation: Bagheri-Khoulenjani S ( Biomedical Engineering Department, Amirkabir University of Technology, Tehran, Iran.)
Abstract	In this study, nanocomposite microspheres based on chitosan/gelatin/nanohydroxyapatite were fabricated, and effects of the nanohydroxyapatite/biopolymer (chitosan/gelatin) weight ratio (nHA/P), stirring rate, chitosan concentration and biopolymer concentration on the particle size, and morphology of nanocomposite microspheres were investigated. Particle size of microspheres was modeled by design of experiments using the surface response method. Particle size, morphology of microspheres, and distribution of nanoparticles within the composite microspheres were evaluated using an optical microscope, scanning electron microscopy (SEM) and transmission electron microscopy (TEM), respectively. X-ray diffraction and Fourier transform infrared spectroscopy were applied to study the physical and chemical characteristics of microspheres. Results showed that by modulating the nHA/P ratio, chitosan concentration, polymer concentration, and stirring rate, it is possible to fabricate microspheres in wide rages of particle size (5-150 µm). Analysis of variance confirmed that the modified quadratic model can be used to predict the particle size of nanocomposite microspheres within the design space. SEM studies showed that microspheres with different compositions had totally different morphologies from dense morphologies to porous ones. TEM images demonstrated that nanoparticles were distributed uniformly within the polymeric matrix. MTT assay and cell culture studies showed that microspheres with different compositions possessed good biocompatibility. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.
File Format	HTM / HTML
ISSN	15493296
Issue Number	6
Volume Number	101
e-ISSN	15524965
Journal	Journal of Biomedical Materials Research Part A
Language	English
Publisher	Wiley
Publisher Date	2013-06-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Durapatite X-ray Diffraction Cell Survival Humans Pharmacology Models, Statistical Spectroscopy, Fourier Transform Infrared Drug Effects Journal Article Discipline Biomedical Engineering Particle Size Microspheres Chemistry Nanocomposites Gelatin Ultrastructure Bone And Bones Cell Line, Tumor Chitosan Methods Tissue Engineering
Content Type	Text
Resource Type	Article
Subject	Ceramics and Composites Metals and Alloys Biomaterials Biomedical Engineering

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