NDLI: Oligodendrocyte differentiation from adult multipotent stem cells is modulated by glutamate.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Cavaliere, F. Urra, O. Alberdi, E. Matute, C.
Description	Country affiliation: Spain Author Affiliation: Cavaliere F ( Department of Neurosciences and CIBERNED, University of Basque Country-UPV/EHU, E-48940 Leioa, Spain.)
Abstract	We used multipotent stem cells (MSCs) derived from the young rat subventricular zone (SVZ) to study the effects of glutamate in oligodendrocyte maturation. Glutamate stimulated oligodendrocyte differentiation from SVZ-derived MSCs through the activation of specific N-methyl-D-aspartate (NMDA) receptor subunits. The effect of glutamate and NMDA on oligodendrocyte differentiation was evident in both the number of newly generated oligodendrocytes and their morphology. In addition, the levels of NMDAR1 and NMDAR2A protein increased during differentiation, whereas NMDAR2B and NMDAR3 protein levels decreased, suggesting differential expression of NMDA receptor subunits during maturation. Microfluorimetry showed that the activation of NMDA receptors during oligodendrocyte differentiation elevated cytosolic calcium levels and promoted myelination in cocultures with neurons. Moreover, we observed that stimulation of MSCs by NMDA receptors induced the generation of reactive oxygen species (ROS), which were negatively modulated by the NADPH inhibitor apocynin, and that the levels of ROS correlated with the degree of differentiation. Taken together, these findings suggest that ROS generated by NADPH oxidase by the activation of NMDA receptors promotes the maturation of oligodendrocytes and favors myelination.
File Format	HTM / HTML
e-ISSN	20414889
DOI	10.1038/cddis.2011.144
Journal	Cell Death and Disease
Volume Number	3
Language	English
Publisher	Nature Publishing Group
Publisher Date	2012-02-02
Publisher Place	Great Britain (UK)
Access Restriction	Open
Subject Keyword	Acetophenones Research Support, Non-u.s. Gov't Reactive Oxygen Species Calcium Coculture Techniques Cytology Antagonists & Inhibitors Multipotent Stem Cells Genetics Cell Differentiation Animals, Newborn Adult Stem Cells Signal Transduction Neurons Cells, Cultured Gene Expression Regulation Rats, Sprague-dawley Receptors, N-methyl-d-aspartate Pharmacology Metabolism Drug Effects Glutamic Acid Discipline Cell Biology Animals Myelin Sheath Nadph Oxidase Protein Isoforms Oligodendroglia
Content Type	Text
Resource Type	Article

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