NDLI: Fabrication and characterization of scaffold from cadaver goat-lung tissue for skin tissue engineering applications.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Gupta, Sweta K. Dinda, Amit K. Potdar, Pravin D. Mishra, Narayan C.
Description	Country affiliation: India Author Affiliation: Gupta SK ( Department of Polymer and Process Engineering, Indian Institute of Technology, Roorkee, India.)
Abstract	The present study aims to fabricate scaffold from cadaver goat-lung tissue and evaluate it for skin tissue engineering applications. Decellularized goat-lung scaffold was fabricated by removing cells from cadaver goat-lung tissue enzymatically, to have cell-free 3D-architecture of natural extracellular matrix. DNA quantification assay and Hematoxylin and eosin staining confirmed the absence of cellular material in the decellularized lung-tissue. SEM analysis of decellularized scaffold shows the intrinsic porous structure of lung tissue with well-preserved pore-to-pore interconnectivity. FTIR analysis confirmed non-denaturation and well maintainance of collagenous protein structure of decellularized scaffold. MTT assay, SEM analysis and H&E staining of human skin-derived Mesenchymal Stem cell, seeded over the decellularized scaffold, confirms stem cell attachment, viability, biocompatibility and proliferation over the decellularized scaffold. Expression of Keratin18 gene, along with CD105, CD73 and CD44, by human skin-derived Mesenchymal Stem cells over decellularized scaffold signifies that the cells are viable, proliferating and migrating, and have maintained their critical cellular functions in the presence of scaffold. Thus, overall study proves the applicability of the goat-lung tissue derived decellularized scaffold for skin tissue engineering applications.
File Format	HTM / HTML
ISSN	09284931
Issue Number	7
Volume Number	33
e-ISSN	18730191
Journal	Materials Science and Engineering: C
Language	English
Publisher	Elsevier
Publisher Date	2013-10-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Materials Science Lung Cytology Skin Tissue Engineering Methods Tissue Scaffolds Chemistry Animals Cadaver Cell Adhesion Cell Proliferation Cell Survival Dna Metabolism Gene Expression Profiling Gene Expression Regulation Goats Humans Ultrastructure Mesenchymal Stromal Cells Reproducibility Of Results Spectroscopy, Fourier Transform Infrared Staining And Labeling Sterilization Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Mechanics of Materials Biomaterials Condensed Matter Physics Bioengineering Mechanical Engineering

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