NDLI: Microcontact imprinted surface plasmon resonance sensor for myoglobin detection.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Osman, Bilgen Uzun, Lokman Besirli, Necati Denizli, Adil
Description	Country affiliation: Turkey Author Affiliation: Osman B ( Uludag University, Department of Chemistry, Bursa, Turkey.)
Abstract	In this study, we prepared surface plasmon resonance (SPR) sensor using the molecular imprinting technique for myoglobin detection in human serum. For this purpose, we synthesized myoglobin imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-l-tryptophan methyl ester) [poly(HEMA-MATrp)] nanofilm on the surface of SPR sensor. We also synthesized non-imprinted poly(HEMA-MATrp) nanofilm without myoglobin for the control experiments. The SPR sensor was characterized with contact angle measurements, atomic force microscopy, X-ray photoelectron spectroscopy, and ellipsometry. We investigated the effectiveness of the sensor using the SPR system. We evaluated the ability of SPR sensor to sense myoglobin with myoglobin solutions (pH7.4, phosphate buffer) in different concentration range and in the serum taken from a patient with acute myocardial infarction. We found that the Langmuir adsorption model was the most suitable for the sensor system. The detection limit was 87.6 ng/mL. In order to show the selectivity of the SPR sensor, we investigated the competitive detection of myoglobin, lysozyme, cytochrome c and bovine serum albumin. The results showed that the SPR sensor has high selectivity and sensitivity for myoglobin.
File Format	HTM / HTML
ISSN	09284931
Issue Number	7
Volume Number	33
e-ISSN	18730191
Journal	Materials Science and Engineering: C
Language	English
Publisher	Elsevier
Publisher Date	2013-10-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Materials Science Molecular Imprinting Instrumentation Methods Myoglobin Analysis Surface Plasmon Resonance Animals Cattle Computer Systems Humans Kinetics Recycling Serum Albumin, Bovine Surface Properties Temperature Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Mechanics of Materials Biomaterials Condensed Matter Physics Bioengineering Mechanical Engineering

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