NDLI: Disposable urea biosensor based on nanoporous ZnO film fabricated from omissible polymeric substrate.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Rahmanian, Reza Mozaffari, Sayed Ahmad Abedi, Mohammad
Description	Country affiliation: Iran Author Affiliation: Rahmanian R ( Thin Layer and Nanotechnology Laboratory, Institute of Chemical Technology, Iranian Research Organization for Science and Technology (IROST), P.O. Box 33535-111, Tehran, Iran.); Mozaffari SA ( Thin Layer and Nanotechnology Laboratory, Institute of Chemical Technology, Iranian Research Organization for Science and Technology (IROST), P.O. Box 33535-111, Tehran, Iran. Electronic address: mozaffari@irost.ir.); Abedi M ( Thin Layer and Nanotechnology Laboratory, Institute of Chemical Technology, Iranian Research Organization for Science and Technology (IROST), P.O. Box 33535-111, Tehran, Iran.)
Abstract	In the present study, a facile and simple fabrication method of a semiconductor based urea biosensor was reported via three steps: (i) producing a ZnO-PVA composite film by means of a polymer assisted electrodeposition of zinc oxide (ZnO) on the F-doped SnO2 conducting glass (FTO) using water soluble polyvinyl alcohol (PVA), (ii) obtaining a nanoporous ZnO film by PVA omission via a subsequent post-treatment by annealing of the ZnO-PVA film, and (iii) preparation of a FTO/ZnO/Urs biosensor by exploiting a nanoporous ZnO film as an efficient and excellent platform area for electrostatic immobilization of urease enzyme (Urs) which was forced by the difference in their isoelectric point (IEP). The characterization techniques focused on the analysis of the ZnO-PVA film surfaces before and after annealing, which had a prominent effect on the porosity of the prepared ZnO film. The surface characterization of the nanostructured ZnO film by a field emission-scanning electron microscopy (FE-SEM), exhibited a film surface area as an effective bio-sensing matrix for enzyme immobilization. The structural characterization and monitoring of the biosensor fabrication was performed using UV-Vis, Fourier Transform Infrared (FT-IR), Raman Spectroscopy, Thermogravimetric Analysis (TGA), Cyclic Voltammetry (CV), and Electrochemical Impedance Spectroscopy (EIS) techniques. The impedimetric results of the FTO/ZnO/Urs biosensor showed a high sensitivity for urea detection within 8.0-110.0mg dL(-1) with the limit of detection as 5.0mg dL(-1).
File Format	HTM / HTML
ISSN	09284931
Volume Number	57
e-ISSN	18730191
Journal	Materials Science and Engineering: C
Language	English
Publisher	Elsevier
Publisher Date	2015-12-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Materials Science Biosensing Techniques Instrumentation Conductometry Metal Nanoparticles Chemistry Nanopores Ultrastructure Urea Blood Zinc Oxide Electrodes Equipment Design Equipment Failure Analysis Humans Reproducibility Of Results Sensitivity And Specificity Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Mechanics of Materials Biomaterials Condensed Matter Physics Bioengineering Mechanical Engineering

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