NDLI: Preparation and characterization of gelatin-hydroxyapatite composite microspheres for hard tissue repair.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Chao, Shao Ching Wang, Ming-Jia Pai, Nai-Su Yen, Shiow-Kang
Description	Author Affiliation: Chao SC ( Department of Materials Science and Engineering, National Chung Hsing University, 250 Kuo-Kuang Road, Taichung 40227, Taiwan); Wang MJ ( Department of Materials Science and Engineering, National Chung Hsing University, 250 Kuo-Kuang Road, Taichung 40227, Taiwan.); Pai NS ( Department of Materials Science and Engineering, National Chung Hsing University, 250 Kuo-Kuang Road, Taichung 40227, Taiwan.); Yen SK ( Department of Materials Science and Engineering, National Chung Hsing University, 250 Kuo-Kuang Road, Taichung 40227, Taiwan. Electronic address: skyen@dragon.nchu.edu.tw.)
Abstract	Gelatin-hydroxyapatite composite microspheres composed of 21% gelatin (G) and 79% hydroxyapatite (HA) with uniform morphology and controllable size were synthesized from a mixed solution of Ca(NO3)2, NH4H2PO4 and gelatin by a wet-chemical method. Material analyses such as X-ray diffraction (XRD), scanning/transmission electron microscopy examination (SEM/TEM) and inductively coupled plasma-mass spectroscopy (ICP-MS) were used to characterize G-HA microspheres by analyzing their crystalline phase, microstructure, morphology and composition. HA crystals precipitate along G fibers to form nano-rods with diameters of 6-10nm and tangle into porous microspheres after blending. The cell culture indicates that G-HA composite microspheres without any toxicity could enhance the proliferation and differentiation of osteoblast-like cells. In a rat calvarial defect model, G-HA bioactive scaffolds were compared with fibrin glue (F) and Osteoset® Bone Graft Substitute (OS) for their capacity of regenerating bone. Four weeks post-implantation, new bone, mineralization, and expanded blood vessel area were found in G-HA scaffolds, indicating greater osteoconductivity and bioactivity than F and OS.
File Format	HTM / HTML
ISSN	09284931
Volume Number	57
e-ISSN	18730191
Journal	Materials Science and Engineering: C
Language	English
Publisher	Elsevier
Publisher Date	2015-12-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Materials Science Bone Substitutes Chemical Synthesis Therapeutic Use Durapatite Chemistry Gelatin Microspheres Skull Fractures Therapy Animals Bone Regeneration Physiology Fracture Healing Materials Testing Rats Rats, Sprague-dawley Pathology Physiopathology Treatment Outcome Journal Article
Content Type	Text
Resource Type	Article
Subject	Mechanics of Materials Biomaterials Condensed Matter Physics Bioengineering Mechanical Engineering

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