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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Chaouch, Mohamed Aymen Hafsa, Jawhar Rihouey, Christophe Le Cerf, Didier Majdoub, Hatem |
| Description | Country affiliation: Tunisia Author Affiliation: Chaouch MA ( Laboratoire des Interfaces et des Matériaux Avancés (LIMA), Faculté des Sciences de Monastir, Université de Monastir, Bd. de l'environnement, 5019 Monastir, Tunisia.); Hafsa J ( Laboratoire de Biochimie, Faculté de Médecine, Université de Sousse, 4002 Sousse, Tunisia.); Rihouey C ( Normandie Université, Laboratoire Polymères Biopolymères Surfaces (PBS), UMR 6270 & FR 3038 CNRS, Université de Rouen, 76821 Mont Saint Aignan, France.); Le Cerf D ( Normandie Université, Laboratoire Polymères Biopolymères Surfaces (PBS), UMR 6270 & FR 3038 CNRS, Université de Rouen, 76821 Mont Saint Aignan, France.); Majdoub H ( Laboratoire des Interfaces et des Matériaux Avancés (LIMA), Faculté des Sciences de Monastir, Université de Monastir, Bd. de l'environnement, 5019 Monastir, Tunisia. Electronic address: hatemmajdoub.fsm@gmail.com.) |
| Abstract | The extraction, purification and degradation of polysaccharides from Opuntia ficus indica cladodes, as well as the evaluation of their antioxidant and antiglycated activities in vitro were investigated. The optimization of the extraction showed that extraction by ultrasound at 40 °C presented the best carbohydrates yield. The degradation of the extracted polysaccharides was achieved by free radical depolymerization with H2O2 in the presence of copper(II) acetate for various reaction times. Sugar contents were determined by colorimetric assays. The macromolecular characteristics of the different isolated and degraded carbohydrates were carried by size exclusion chromatography (SEC/MALS/VD/DRI). These experiments showed that all samples are polysaccharides, which are probably pectins and that molecular weight (Mw) has decreased from 6,800,000 to 14,000 g/mol after 3 h of depolymerization without changing the structure. Preliminary antioxidant and antiglycated tests indicated that degraded polysaccharides for 2 and 3 h showed even better antioxidant and antiglycated activities. |
| File Format | HTM / HTML |
| ISSN | 01418130 |
| Volume Number | 79 |
| e-ISSN | 18790003 |
| Journal | International Journal of Biological Macromolecules |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-08-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry Antioxidants Chemistry Opuntia Pectins Animals Isolation & Purification Biphenyl Compounds Antagonists & Inhibitors Cattle Chromatography, Gel Glycosylation Hydrogen Peroxide Linoleic Acid Lipid Peroxidation Organometallic Compounds Picrates Plant Stems Serum Albumin, Bovine Solutions Sonication Temperature Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Structural Biology Molecular Biology Biochemistry |
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