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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Sachdeva, Veena Hooda, Vinita |
| Description | Country affiliation: India Author Affiliation: Sachdeva V ( Department of Botany, Faculty of Life Sciences, Maharshi Dayanand University, Rohtak 124001, India.); Hooda V ( Department of Botany, Faculty of Life Sciences, Maharshi Dayanand University, Rohtak 124001, India.) |
| Abstract | Epoxy glued silver nanoparticles were used as immobilization support for nitrate reductase (NR). The resulting epoxy/AgNPs/NR conjugates were characterized at successive stages of fabrication by scanning electron microscopy and fourier transform infrared spectroscopy. The immobilized enzyme system exhibited reasonably high conjugation yield (37.6±0.01 µg/cm(2)), with 93.54±0.88% retention of specific activity. Most favorable working conditions of pH, temperature and substrate concentration were ascertained to optimize the performance of epoxy/AgNPs/NR conjugates for soil nitrate quantification. The analytical results for soil nitrate determination were consistent, reliable and reproducible. Minimum detection limit of the method was 0.05 mM with linearity from 0.1 to 11.0 mM. The % recoveries of added nitrates (0.1 and 0.2 mM) were<95.0% and within-day and between-day coefficients of variations were 0.556% and 1.63% respectively. The method showed good correlation (R(2)=0.998) with the popular Griess reaction method. Epoxy/AgNPs bound NR had a half-life of 18 days at 4 °C and retained 50% activity after 15 reuses. |
| File Format | HTM / HTML |
| ISSN | 01418130 |
| Volume Number | 79 |
| e-ISSN | 18790003 |
| Journal | International Journal of Biological Macromolecules |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-08-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry Enzymes, Immobilized Chemistry Metal Nanoparticles Nitrate Reductase Nitrates Isolation & Purification Silver Soil Pollutants Soil Adsorption Epoxy Resins Equipment Reuse Hydrogen-ion Concentration Limit Of Detection Ultrastructure Reproducibility Of Results Temperature Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Structural Biology Molecular Biology Biochemistry |
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