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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Chakraborty, Samrat Ghosh, Mandakini Chakraborti, Srijita Jana, Sougata Sen, Kalyan Kumar Kokare, Chandrakant Zhang, Lixin |
| Description | Country affiliation: India Author Affiliation: Chakraborty S ( Department of Pharmaceutics, Gupta College of Technological Sciences, Asansol 713301, WB, India.); Ghosh M ( Department of Pharmaceutics, Gupta College of Technological Sciences, Asansol 713301, WB, India.); Chakraborti S ( Department of Pharmaceutics, Gupta College of Technological Sciences, Asansol 713301, WB, India.); Jana S ( Department of Pharmaceutics, Gupta College of Technological Sciences, Asansol 713301, WB, India. Electronic address: janapharmacy@rediffmail.com.); Sen KK ( Department of Pharmaceutics, Gupta College of Technological Sciences, Asansol 713301, WB, India.); Kokare C ( Department of Pharmaceutics, Sinhgad Institute of Pharmacy, Narhe, Pune 411 041, India.); Zhang L ( Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.) |
| Abstract | This investigation aims to isolate an Actinomycetes strain producing a biosurfactant from the unexplored region of industrial and coal mine areas. Actinomycetes are selected for this study as their novel chemistry was not exhausted and they have tremendous potential to produce bioactive secondary metabolites. The biosurfactant was characterized and further needed to be utilized for pharmaceutical dosage form. Isolation, purification, screening, and characterization of the Actinomycetes A17 were done followed by its fermentation in optimized conditions. The cell-free supernatant was used for the extraction of the biosurfactant and precipitated by cold acetone. The dried precipitate was purified by TLC and the emulsification index, surface tension and CMC were determined. The isolated strain with preferred results was identified as Actinomycetes nocardiopsis A17 with high foam-forming properties. It gives lipase, amylase, gelatinase, and protease activity. The emulsification index was found to be 93±0.8 with surface tension 66.67 dyne/cm at the lowest concentration and cmc 0.6 µg/ml. These biosurfactants were characterized by Fourier transform infra red (FT-IR) spectroscopy and liquid chromatography-mass spectrometry (LC-MS). Therefore, it can be concluded that the biosurfactant produced by Actinomycetes nocardiopsis sp. strain A17 was found to have satisfactory results with high surface activity and emulsion-forming ability. |
| File Format | HTM / HTML |
| ISSN | 01418130 |
| Volume Number | 79 |
| e-ISSN | 18790003 |
| Journal | International Journal of Biological Macromolecules |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-08-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry Actinobacteria Metabolism Rna, Ribosomal, 16s Genetics Surface-active Agents Chemistry Classification Amylases Biosynthesis Isolation & Purification Emulsions Fermentation Gelatinases Hydrogen-ion Concentration Lipase Micelles Peptide Hydrolases Phylogeny Surface Tension Temperature Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Structural Biology Molecular Biology Biochemistry |
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