NDLI: In-depth sequence analysis of highly-conserved pyrH gene to study distributions of oral treponemes in periodontal disease versus health

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In-depth sequence analysis of highly-conserved pyrH gene to study distributions of oral treponemes in periodontal disease versus health

Content Provider	Taylor & Francis Online
Author	Watt, Rory M.
Abstract	Background: More than 75 species/species-level phylotypes of oral treponeme bacteria inhabit the oral cavity. However, their respective genomic compositions and clinical distributions remain poorly understood.Objectives: To compare distributions of phylogroup 1 and 2 oral treponemes in subjects with various periodontal health conditions, via sequence analysis of a highly-conserved treponeme ‘housekeeping’ gene.Methods: Subgingival plaque samples were collected from Chinese subjects with chronic periodontitis (n=5), aggressive periodontitis (n=4), gingivitis (n=5), and healthy controls (n=4). Samples were analyzed by a PCR/plasmid clone sequencing-based approach, using primer sets targeting the pyrH gene. Data was analyzed using various computational/bioinformatic approaches.Results: 1,227 quality-filtered pyrH gene sequences were obtained (mean 66.2±9.6 sequences per subject), which were assigned to 33 ‘pyrH genotypes’ (97% identity cut-off). 538 pyrH sequences (17 pyrH genotypes) corresponded to phylogroup 1 treponemes (including ‘T. vincentii’, Treponema medium, and ‘Treponema sinensis’ taxa). 689 pyrH sequences (16 pyrH genotypes) corresponded to phylogroup 2 taxa. Correlations between pyrH genotype distributions and disease status were complex. One pyrH genotype, which was phylogenetically-related to T. denticola GM-1/MS25 strains, was highly prevalent: being detected in 17/18 subjects.Conclusions: Both healthy and periodontally-diseased subjects harbor multiple genetic lineages corresponding to the same treponeme species/phylotype within their subgingival niches.
File Format	PDF HTM / HTML
ISSN	20002297
DOI	10.1080/20002297.2017.1325210
Journal	Journal of Oral Microbiology
Volume Number	9
Issue Number	sup1
Language	English
Publisher	Taylor & Francis
Publisher Date	2017-06-01
Access Restriction	Open
Content Type	Text
Subject	Microbiology (medical) Dentistry Infectious Diseases 3500/3501

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