NDLI: Investigation of fitness cost, copy number variation and excision rate of Tn916/Tn916-like elements in oral streptococci and enterococci

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Investigation of the prevalence of Tn916/Tn916-like elements in oral streptococci

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Investigation of fitness cost, copy number variation and excision rate of Tn916/Tn916-like elements in oral streptococci and enterococci

Content Provider	Taylor & Francis Online
Author	Al-Haroni, Mohammed Lunde, Tracy Roberts, Adam
Abstract	The Tn916 and other related elements are broad host range conjugative transposons that are widely spread in bacteria. These elements contribute to the spread of antibiotic resistance genes (ARG) between bacterial species. Variation in the Tn916/Tn916-like elements copy number (Tn916CN) and their excision frequency (Tn916ExFr) from their host genome could play a role in propagation of ARG. In this study, the Tn916CN and the Tn916ExFr in oral streptococci and enterococci were determined using droplet digital PCR (ddPCR). In addition, we investigated the fitness cost associated with new acquisition of Tn916 in a selected oral streptococci and enterococci strains. Amelioration of fitness cost and mechanism(s) to mitigate fitness loss were evaluated by ddPCR and next generation sequencing (NGS). We found the ddPCR is superior to southern blot to determine Tn916CN. It was also found that both the excision rate of Tn916 and its autonomous replication increase because of antimicrobial challenge. Loss of bacterial fitness was usually associated with multiple acquisitions of Tn916. It is concluded that amelioration of fitness cost is achieved after a few hundred generations and by maintaining one copy of Tn916 in bacterial genome under no selection pressure.
File Format	PDF HTM / HTML
ISSN	20002297
DOI	10.1080/20002297.2017.1325191
Journal	Journal of Oral Microbiology
Volume Number	9
Issue Number	sup1
Language	English
Publisher	Taylor & Francis
Publisher Date	2017-06-09
Access Restriction	Open
Content Type	Text
Subject	Microbiology (medical) Dentistry Infectious Diseases 3500/3501

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Investigation of the prevalence of Tn916/Tn916-like elements in oral streptococci

Determination of copy number and circularization ratio of Tn916-Tn1545 family of conjugative transposons in oral streptococci by droplet digital PCR

Prevalence, diversity and transferability of the Tn916-Tn1545 family ICE in oral streptococci.

Natural occurrence of structures in oral streptococci and enterococci with DNA homology to Tn916.

Variation on a theme; an overview of the Tn916/Tn1545 family of mobile genetic elements in the oral and nasopharyngeal streptococci

Incidence and behaviour of Tn916-like elements within tetracycline-resistant bacteria isolated from root canals

Transfer of Tn916-Like Elements in Microcosm Dental Plaques

Virulence factors and antibiotic susceptibility in enterococci isolated from oral mucosal and deep infections

Virulence factors and antibiotic susceptibility in enterococci isolated from oral mucosal and deep infections

Investigation of fitness cost, copy number variation and excision rate of Tn916/Tn916-like elements in oral streptococci and enterococci

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