NDLI: Metataxonomics study of dental bioaerosols affected by waterline disinfection

Content Provider	Springer Nature : BioMed Central
Author	Poolkerd, Wachirakorn Swatasuk, Boonlased Saengpitak, Methanee Muangsawat, Sureeporn Klankeo, Piriya Thotsaporn, Kittisak Ampornaramveth, Ruchanee Salingcarnboriboon
Abstract	Background Microorganisms in dental unit water (DUW) play a significant role in dental bioaerosols. If the methods used to decontaminate DUW also help improve air quality in dental clinics is worth exploring. In this study, we aim to identify the source of bacteria in dental bioaerosols and investigate the impact of waterline disinfectants on the quantity and composition of bacteria in DUW and bioaerosols. Methods Two dental chair units in a separate treatment room are installed with two different waterline decontamination systems, a plasma or iodine cartridge. The experiment was performed in two phases, before and after installing the decontamination systems. Aerosol is generated via running airotor in the subject’s mouth. Before and after the procedure, the air samples were collected with an active air sampling machine onto agar plate and filter paper for genomic DNA extraction. The subject’s saliva and DUW samples were also collected. The samples were analyzed further with bacterial counting and metataxonomics analysis. Results The bacteria present in the air sample after the aerosol-generating procedure were confirmed to be derived from the air-before, saliva, and DUW in 51.43%, 6.38%, and 18.60%, respectively. The saliva samples demonstrated the highest alpha diversity (within the sample), whereas the air samples had the least. Both waterline disinfectants effectively controlled bacteria in DUW but did not affect the bacterial number and composition in the air. Conclusions Dental bioaerosols are composed of bacteria from saliva and DUW. Plasma and iodine showed a trend in controlling bacterial contamination in DUW but did not alter the bacterial count and composition in dental bioaerosols.
Related Links	https://bmcoralhealth.biomedcentral.com/counter/pdf/10.1186/s12903-024-05304-4.pdf
Ending Page	9
Page Count	9
Starting Page	1
File Format	HTM / HTML
ISSN	14726831
DOI	10.1186/s12903-024-05304-4
Journal	BMC Oral Health
Issue Number	1
Volume Number	24
Language	English
Publisher	BioMed Central
Publisher Date	2024-12-31
Access Restriction	Open
Subject Keyword	Dentistry Oral and Maxillofacial Surgery Dental unit waterline decontamination Dental bioaerosols Iodine Plasma Infection control
Content Type	Text
Resource Type	Article
Subject	Dentistry
Journal Impact Factor	2.6/2023
5-Year Journal Impact Factor	3.2/2023

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Dental unit waterline testing practices: an 11-Year retrospective study

Comparative evaluation of effect of sodium hypochlorite and chlorhexidine in dental unit waterline on aerosolized bacteria generated during dental treatment

Bacterial biofilm prevalence in dental unit waterlines: a systematic review and meta-analysis

Effect of nanoemulsion on dental unit waterline biofilm

Practices of Infection Control Among Dental Care Providers: A Cross Sectional Study

Waterline Disinfectants Reduce Dental Bioaerosols: A Multitracer Validation

3 centre of dentistry, oral.

Assessment of dental clinics’ infection control function using a checklist during the COVID-19 pandemic

Analysis of sterilizer allocation and related factors in dental health-care settings in Yunnan Province, China: a cross-sectional study

Metataxonomics study of dental bioaerosols affected by waterline disinfection

Similar Documents

Dental unit waterline testing practices: an 11-Year retrospective study

Comparative evaluation of effect of sodium hypochlorite and chlorhexidine in dental unit waterline on aerosolized bacteria generated during dental treatment

Bacterial biofilm prevalence in dental unit waterlines: a systematic review and meta-analysis

Effect of nanoemulsion on dental unit waterline biofilm

Practices of Infection Control Among Dental Care Providers: A Cross Sectional Study

Waterline Disinfectants Reduce Dental Bioaerosols: A Multitracer Validation

3 centre of dentistry, oral.

Assessment of dental clinics’ infection control function using a checklist during the COVID-19 pandemic

Analysis of sterilizer allocation and related factors in dental health-care settings in Yunnan Province, China: a cross-sectional study

Metataxonomics study of dental bioaerosols affected by waterline disinfection