NDLI: Expression patterns of mechanosensitive ion channel PIEZOs in irreversible pulpitis

Content Provider	Springer Nature : BioMed Central
Author	Yang, Wenying Lin, Lu Hu, Shucheng Jiang, Bin Yang, Ruhan Yu, Weijun Tang, Jiaqi Zhao, Dan Gu, Yuting Jin, Min Li, Jin Lu, Eryi
Abstract	Background Mechanosensitive ion channel PIEZOs have been widely reported to involve inflammation and pain. This study aimed to clarify expression patterns of PIEZOs and their potential relations to irreversible pulpitis. Materials and methods Normal pulp tissues (n = 29) from patients with impacted third molars and inflamed pulp tissues (n = 23) from patients with irreversible pulpitis were collected. Pain levels were assessed using a numerical rating scale. PIEZO expressions were measured using real-time PCR and then confirmed using GEO datasets GSE77459, immunoblot, and immunohistochemistry staining. Correlations of PIEZO mRNA expression with inflammatory markers, pain markers, or clinical pain levels were evaluated using Spearman’s correlation analysis. Univariate analysis was conducted to analyze PIEZO expressions based on pain description and clinical examinations of cold test, percussion, palpation, and bite test. Results Compared with normal pulp tissues, mRNA expression levels of PIEZO1 were significantly increased in inflamed pulp tissues, while PIEZO2 was significantly decreased, which was further confirmed in GSE77459 and on a protein and histological level. The positive correlation of the mRNA expression levels between PIEZO1 and inflammatory markers, as well as between PIEZO2 and pain markers, was verified. PIEZO2 expression was also positively correlated with pain levels. Besides, irreversible pulpitis patients who reported continuous pain and who detected a positive response to cold stimulus exhibited a higher expression level of PIEZO2 in the inflamed pulp tissues. By contrast, patients reporting pain duration of more than one week showed a higher expression level of PIEZO1. Conclusions This study demonstrated the upregulation of PIEZO1 and the downregulation of PIEZO2 in irreversible pulpitis and revealed the potential relation of PIEZO1 and PIEZO2 to inflammation and pain. These findings suggested that PIEZOs might play critical roles in the progression of irreversible pulpitis and paved the way for further investigations aimed at novel therapies of irreversible pulpitis by targeting PIEZOs.
Related Links	https://bmcoralhealth.biomedcentral.com/counter/pdf/10.1186/s12903-024-04209-6.pdf
Ending Page	12
Page Count	12
Starting Page	1
File Format	HTM / HTML
ISSN	14726831
DOI	10.1186/s12903-024-04209-6
Journal	BMC Oral Health
Issue Number	1
Volume Number	24
Language	English
Publisher	BioMed Central
Publisher Date	2024-04-16
Access Restriction	Open
Subject Keyword	Dentistry Oral and Maxillofacial Surgery Irreversible pulpitis Mechanosensitive ion channels PIEZO1 PIEZO2 Inflammation Pain
Content Type	Text
Resource Type	Article
Subject	Dentistry
Journal Impact Factor	2.6/2023
5-Year Journal Impact Factor	3.2/2023

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Mechanosensitive Piezo1 and Piezo2 ion channels in craniofacial development and dentistry: Recent advances and prospects

The prevalence of dental anxiety and its association with pain and other variables among adult patients with irreversible pulpitis

Identification and validation of m6A RNA regulatory network in pulpitis

The mechanosensitive ion channel Piezo2 mediates sensitivity to mechanical pain in mice

Antibiotic use for irreversible pulpitis

The mechanosensitive ion channel Piezo1 contributes to ultrasound neuromodulation.

Mechanosensitive ion channels in apoptosis and ferroptosis: focusing on the role of Piezo1.

2021 Nobel Prize for mechanosensory transduction.

Piezo1 Channels Are Inherently Mechanosensitive

Expression patterns of mechanosensitive ion channel PIEZOs in irreversible pulpitis

Similar Documents

Mechanosensitive Piezo1 and Piezo2 ion channels in craniofacial development and dentistry: Recent advances and prospects

The prevalence of dental anxiety and its association with pain and other variables among adult patients with irreversible pulpitis

Identification and validation of m6A RNA regulatory network in pulpitis

The mechanosensitive ion channel Piezo2 mediates sensitivity to mechanical pain in mice

Antibiotic use for irreversible pulpitis

The mechanosensitive ion channel Piezo1 contributes to ultrasound neuromodulation.

Mechanosensitive ion channels in apoptosis and ferroptosis: focusing on the role of Piezo1.

2021 Nobel Prize for mechanosensory transduction.

Piezo1 Channels Are Inherently Mechanosensitive

Expression patterns of mechanosensitive ion channel PIEZOs in irreversible pulpitis