NDLI: Lactucaxanthin – a potential anti-diabetic carotenoid from lettuce (<i>Lactuca sativa</i>) inhibits α-amylase and α-glucosidase activity <i>in vitro</i> and in diabetic rats

Please wait, while we are loading the content...

Olive leaf extract concentrated in hydroxytyrosol attenuates protein carbonylation and the formation of advanced glycation end products in a hepatic cell line (HepG2)

Dietary 5-demethylnobiletin inhibits cigarette carcinogen NNK-induced lung tumorigenesis in mice

Dietary polyacetylenes, falcarinol and falcarindiol, isolated from carrots prevents the formation of neoplastic lesions in the colon of azoxymethane-induced rats

Contribution of the phenolic composition to the antioxidant, anti-inflammatory and antitumor potential of Equisetum giganteum L. and Tilia platyphyllos Scop.

Rats’ urinary metabolomes reveal the potential roles of functional foods and exercise in obesity management

Activation of Nrf2-driven antioxidant enzymes by cardamonin confers neuroprotection of PC12 cells against oxidative damage

Negative effects of divalent mineral cations on the bioaccessibility of carotenoids from plant food matrices and related physical properties of gastro-intestinal fluids

Immunomodulatory effects of Hericium erinaceus derived polysaccharides are mediated by intestinal immunology

Changes of structure and IgE binding capacity of shrimp (Metapenaeus ensis) tropomyosin followed by acrolein treatment

The impact of solvent relative permittivity on the dimerisation of organic molecules well below their solubility limits: examples from brewed coffee and beyond

Proanthocyanidins purified from fruit pericarp of Clausena lansium (Lour.) Skeels as efficient tyrosinase inhibitors: structure evaluation, inhibitory activity and molecular mechanism

Identification of terpenoids from Rubus corchorifolius L. f. leaves and their anti-proliferative effects on human cancer cells

Formononetin inhibits human bladder cancer cell proliferation and invasiveness via regulation of miR-21 and PTEN

Garcinol sensitizes breast cancer cells to Taxol through the suppression of caspase-3/iPLA₂ and NF-κB/Twist1 signaling pathways in a mouse 4T1 breast tumor model

Creation and imitation of a milk flavour

Impact of liposomal encapsulation on degradation of anthocyanins of black carrot extract by adding ascorbic acid

Sialoglycoprotein isolated from the eggs of Gadus morhua enhances fracture healing in osteoporotic mice

Preventive effect of Silibinin in combination with Pu-erh tea extract on non-alcoholic fatty liver disease in ob/ob mice

A potential sex dimorphism in the relationship between bitter taste and alcohol consumption

Lactucaxanthin – a potential anti-diabetic carotenoid from lettuce (Lactuca sativa) inhibits α-amylase and α-glucosidase activity in vitro and in diabetic rats

The anti-obesity effect of fermented barley extracts with Lactobacillus plantarum dy-1 and Saccharomyces cerevisiae in diet-induced obese rats

Collagen peptides ameliorate intestinal epithelial barrier dysfunction in immunostimulatory Caco-2 cell monolayers via enhancing tight junctions

Shedding light on the biological and chemical fingerprints of three Achillea species (A. biebersteinii, A. millefolium and A. teretifolia)

Dietary fibre-based SCFA mixtures promote both protection and repair of intestinal epithelial barrier function in a Caco-2 cell model

Kodo millet whole grain and bran supplementation prevents high-fat diet induced derangements in a lipid profile, inflammatory status and gut bacteria in mice

Chemical characterization of the glycated myofibrillar proteins from grass carp (Ctenopharyngodon idella) and their impacts on the human gut microbiota in vitro fermentation

Ara h 2 cross-linking catalyzed by MTGase decreases its allergenicity

Metabonomic analysis of quercetin against the toxicity of acrylamide in rat urine

Beneficial effects of Lycium barbarum polysaccharide on spermatogenesis by improving antioxidant activity and inhibiting apoptosis in streptozotocin-induced diabetic male mice

In vitro genotoxicity of carcinogenic asarone isomers

Protective effect of isoliquiritin against corticosterone-induced neurotoxicity in PC12 cells

Molecular mechanisms of the effects of the ethanolic extract of Muntingia calabura Linn. fruit on lipopolysaccharide-induced pro-inflammatory mediators in macrophages

Transcriptomic analyses of the anti-adipogenic effects of oleuropein in human mesenchymal stem cells

Effects of dietary glucocerebrosides from sea cucumber on the brain sphingolipid profiles of mouse models of Alzheimer's disease

Beneficial effects of apple peel polyphenols on vascular endothelial dysfunction and liver injury in high choline-fed mice

A diet containing a high- versus low-daidzein level does not protect against liver steatosis in the obese Zucker rat model

Chemical characterization of a novel polysaccharide ASKP-1 from Artemisia sphaerocephala Krasch seed and its macrophage activation via MAPK, PI3k/Akt and NF-κB signaling pathways in RAW264.7 cells

Flavonoids from sea buckthorn inhibit the lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages through the MAPK and NF-κB pathways

Neuroprotective effects of phytosterol esters against high cholesterol-induced cognitive deficits in aged rat

The colonic metabolites dihydrocaffeic acid and dihydroferulic acid are more effective inhibitors of in vitro platelet activation than their phenolic precursors

Back cover

Food & Function Vol - 8 Issue - 2

Food & Function Vol - 8 Issue - 1

Food & Function Vol -7

Food & Function Vol -6

Food & Function Vol -5

Food & Function Vol -4

Food & Function Vol -3

Food & Function Vol -2

Food & Function Vol -1

Lactucaxanthin – a potential anti-diabetic carotenoid from lettuce (Lactuca sativa) inhibits α-amylase and α-glucosidase activity in vitro and in diabetic rats

Content Provider	Royal Society of Chemistry (RSC)
Author	Lakshmi, Magisetty Jhansi Sharavana, Gurunathan Sathaiah, Gunaseelan Gopal, Sowmya Shree Sreerama, Yadahally N Baskaran, Vallikannan
Copyright Year	2017
Abstract	Intestinal and pancreatic α-amylase and α-glucosidase inhibitors offer an approach to lower the levels of post-prandial hyperglycemia through the control of dietary starch breakdown in digestion. This study hypothesized that lactucaxanthin (Lxn) in lettuce (Lactuca sativa) inhibits the activity of α-amylase and α-glucosidase. In this study, the interaction of Lxn with α-amylase and α-glucosidase in silico and its inhibitory effect on these enzymes were studied using in vitro and STZ-induced diabetic rat models. Lxn was isolated from lettuce with 96% purity confirmed by HPLC and LCMS. The in silico analysis showed that Lxn has a lower binding energy (−6.05 and −6.34 kcal mol−1) with α-amylase and α-glucosidase compared to their synthetic inhibitors, acarbose (−0.21 kcal mol−1) and miglitol (−2.78 kcal mol−1), respectively. In vitro α-amylase and α-glucosidase inhibition assays revealed that Lxn had IC50 values of 435.5 μg mL−1 and 1.84 mg mL−1, but acarbose has values of 2.5 and 16.19 μg mL−1. The in vivo results showed an increased activity for α-amylase and α-glucosidase in the intestine (4.7 and 1.30 fold, p < 0.05) and pancreas (1.3 and 1.48 fold, p < 0.05) of STZ induced diabetic rats compared to normal rats. Whereas the activity decreased (p < 0.05) in the Lxn fed diabetic rats, except for the intestinal α-glucosidase activity (1.69 ± 0.12 PNP per min per mg protein). This was confirmed by the low blood glucose level (239.4 ± 18.2 mg dL−1) in diabetic rats fed Lxn compared to the diabetic group (572.2 ± 30.5 mg dL−1, p < 0.05). Lxn significantly inhibited (p < 0.05) the activity of α-amylase and α-glucosidase and could be of medical and nutritional relevance in the treatment of diabetes.
Starting Page	1124
Ending Page	1131
Page Count	8
File Format	HTM / HTML PDF
ISSN	20426496
Volume Number	8
Issue Number	3
Journal	Food & Function
DOI	10.1039/c6fo01655c
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	HPLC LCMS Lxn Lactuca Alpha-amylase Glycoside hydrolase Hyperglycemia Starch Lettuce High-performance liquid chromatography Binding energy Acarbose Miglitol IC50 Small intestine Pancreas Protein Glucose Diabetes mellitus type 2
Content Type	Text
Resource Type	Article
Subject	Medicine Food Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Lactucaxanthin - a potential anti-diabetic carotenoid from lettuce (Lactuca sativa) inhibits α-amylase and α-glucosidase activity in vitro and in diabetic rats

Inhibitory effects of medicinal mushrooms on α-amylase and α-glucosidase – enzymes related to hyperglycemia

Grape skin phenolics as inhibitors of mammalian α-glucosidase and α-amylase – effect of food matrix and processing on efficacy

Inhibition of α-glucosidase by vitamin D₃ and the effect of vitamins B₁ and B₂

Interaction of antidiabetic α-glucosidase inhibitors and gut bacteria α-glucosidase

Cinnamon extract inhibits α-glucosidase activity and dampens postprandial glucose excursion in diabetic rats

Sargassum yezoense Extract Inhibits Carbohydrate Digestive Enzymes In Vitro and Alleviates Postprandial Hyperglycemia in Diabetic Mice.

Comparative Study Of Effect Of Alpha Glucosidase Inhibitors – Miglitol, Acarbose And Voglibose On Postprandial Hyperglycemia And Glycosylated Hemoglobin In Type-2 Diabetes Mellitus

Polyopes lancifolia Extract, a Potent α-Glucosidase Inhibitor, Alleviates Postprandial Hyperglycemia in Diabetic Mice.

Lactucaxanthin – a potential anti-diabetic carotenoid from lettuce (Lactuca sativa) inhibits α-amylase and α-glucosidase activity in vitro and in diabetic rats

Similar Documents

Lactucaxanthin - a potential anti-diabetic carotenoid from lettuce (Lactuca sativa) inhibits α-amylase and α-glucosidase activity in vitro and in diabetic rats

Inhibitory effects of medicinal mushrooms on α-amylase and α-glucosidase – enzymes related to hyperglycemia

Grape skin phenolics as inhibitors of mammalian α-glucosidase and α-amylase – effect of food matrix and processing on efficacy

Inhibition of α-glucosidase by vitamin D3 and the effect of vitamins B1 and B2

Interaction of antidiabetic α-glucosidase inhibitors and gut bacteria α-glucosidase

Cinnamon extract inhibits α-glucosidase activity and dampens postprandial glucose excursion in diabetic rats

Sargassum yezoense Extract Inhibits Carbohydrate Digestive Enzymes In Vitro and Alleviates Postprandial Hyperglycemia in Diabetic Mice.

Comparative Study Of Effect Of Alpha Glucosidase Inhibitors – Miglitol, Acarbose And Voglibose On Postprandial Hyperglycemia And Glycosylated Hemoglobin In Type-2 Diabetes Mellitus

Polyopes lancifolia Extract, a Potent α-Glucosidase Inhibitor, Alleviates Postprandial Hyperglycemia in Diabetic Mice.

Lactucaxanthin – a potential anti-diabetic carotenoid from lettuce (Lactuca sativa) inhibits α-amylase and α-glucosidase activity in vitro and in diabetic rats

Inhibition of α-glucosidase by vitamin D₃ and the effect of vitamins B₁ and B₂