NDLI: Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity

Content Provider	PubMed Central
Author	Sakuma, Tetsushi Ochiai, Hiroshi Kaneko, Takehito Mashimo, Tomoji Tokumasu, Daisuke Sakane, Yuto Suzuki, Ken-ichi Miyamoto, Tatsuo Sakamoto, Naoaki Matsuura, Shinya Yamamoto, Takashi
Copyright Year	2013
Abstract	Transcription activator-like effector (TALE) nuclease (TALEN) is a site-specific nuclease, which can be freely designed and easily constructed. Numerous methods of constructing TALENs harboring different TALE scaffolds and repeat variants have recently been reported. However, the functionalities of structurally different TALENs have not yet been compared. Here, we report on the functional differences among several types of TALENs targeting the same loci. Using HEK293T cell-based single-strand annealing and Cel-I nuclease assays, we found that TALENs with periodically-patterned repeat variants harboring non-repeat-variable di-residue (non-RVD) variations (Platinum TALENs) showed higher activities than TALENs without non-RVD variations. Furthermore, the efficiencies of gene disruption mediated by Platinum TALENs in frogs and rats were significantly higher than in previous reports. This study therefore demonstrated an efficient system for the construction of these highly active Platinum TALENs (Platinum Gate system), which could establish a new standard in TALEN engineering.
Related Links	http://dx.doi.org/10.1038/srep03379
Starting Page	3379
File Format	PDF
ISSN	20452322
e-ISSN	20452322
Journal	Scientific Reports
Volume Number	3
Language	English
Publisher	Nature Publishing Group
Publisher Date	2013-11-29
Access Restriction	Open
Rights Holder	Nature Publishing Group
Subject Keyword	Science and technology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity

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