NDLI: Cell electrofusion using nanosecond electric pulses

Content Provider	PubMed Central
Author	Rems, Lea Marko, Ušaj Kandušer, Maša Reberšek, Matej Miklavčič, Damijan Pucihar, Gorazd
Copyright Year	2013
Abstract	Electrofusion is an efficient method for fusing cells using short-duration high-voltage electric pulses. However, electrofusion yields are very low when fusion partner cells differ considerably in their size, since the extent of electroporation (consequently membrane fusogenic state) with conventionally used microsecond pulses depends proportionally on the cell radius. We here propose a new and innovative approach to fuse cells with shorter, nanosecond (ns) pulses. Using numerical calculations we demonstrate that ns pulses can induce selective electroporation of the contact areas between cells (i.e. the target areas), regardless of the cell size. We then confirm experimentally on B16-F1 and CHO cell lines that electrofusion of cells with either equal or different size by using ns pulses is indeed feasible. Based on our results we expect that ns pulses can improve fusion yields in electrofusion of cells with different size, such as myeloma cells and B lymphocytes in hybridoma technology.
Related Links	http://dx.doi.org/10.1038/srep03382
Starting Page	3382
File Format	PDF
ISSN	20452322
e-ISSN	20452322
Journal	Scientific Reports
Volume Number	3
Language	English
Publisher	Nature Publishing Group
Publisher Date	2013-11-01
Access Restriction	Open
Rights Holder	Nature Publishing Group
Subject Keyword	Science and technology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Cell electrofusion using nanosecond electric pulses

Impact of external medium conductivity on cell membrane electropermeabilization by microsecond and nanosecond electric pulses

Electrosensitization assists cell ablation by nanosecond pulsed electric field in 3D cultures

Gadolinium modifies the cell membrane to inhibit permeabilization by nanosecond electric pulses

Inactivation of Ricin Toxin by Nanosecond Pulsed Electric Fields Including Evidences from Cell and Animal Toxicity

Bipolar nanosecond electric pulses are less efficient at electropermeabilization and killing cells than monopolar pulses

Electroporation of Intracellular Liposomes Using Nanosecond Electric Pulses—A Theoretical Study

Electroporation of mammalian cells by nanosecond electric field oscillations and its inhibition by the electric field reversal

Transmembrane molecular transport during versus after extremely large, nanosecond electric pulses

Cell electrofusion using nanosecond electric pulses

Similar Documents

Cell electrofusion using nanosecond electric pulses

Impact of external medium conductivity on cell membrane electropermeabilization by microsecond and nanosecond electric pulses

Electrosensitization assists cell ablation by nanosecond pulsed electric field in 3D cultures

Gadolinium modifies the cell membrane to inhibit permeabilization by nanosecond electric pulses

Inactivation of Ricin Toxin by Nanosecond Pulsed Electric Fields Including Evidences from Cell and Animal Toxicity

Bipolar nanosecond electric pulses are less efficient at electropermeabilization and killing cells than monopolar pulses

Electroporation of Intracellular Liposomes Using Nanosecond Electric Pulses—A Theoretical Study

Electroporation of mammalian cells by nanosecond electric field oscillations and its inhibition by the electric field reversal

Transmembrane molecular transport during versus after extremely large, nanosecond electric pulses

Cell electrofusion using nanosecond electric pulses