NDLI: A two-photon ratiometric fluorescence probe for Cupric Ions in Live Cells and Tissues

Content Provider	PubMed Central
Author	Zhu, Anwei Ding, Changqin Tian, Yang
Copyright Year	2013
Abstract	Development of sensitive and selective probes for cupric ions (Cu2+) at cell and tissue level is a challenging work for progress in understanding the biological effects of Cu2+. Here, we report a ratiometric two-photon probe for Cu2+ based on the organic-inorganic hybrids of graphene quantum dots (GQDs) and Nile Blue dye. Meanwhile, Cu-free derivative of copper-zinc superoxide dismutase (SOD) – E2Zn2SOD is designed as the unique receptor for Cu2+ and conjugated on the surface of GQDs. This probe shows a blue-to-yellow color change in repose to Cu2+, good selectivity, low cytotoxicity, long-term photostability, and insensitivity to pH over the biologically relevant pH range. The developed probe allows the direct visualization of Cu2+ levels in live cells as well as in deep-tissues at 90–180 μm depth through the use of two-photon microscopy. Furthermore, the effect of ascorbic acid is also evaluated on intracellular Cu2+ binding to E2Zn2SOD by this probe.
Related Links	http://dx.doi.org/10.1038/srep02933
Starting Page	2933
File Format	PDF
ISSN	20452322
e-ISSN	20452322
Journal	Scientific Reports
Volume Number	3
Language	English
Publisher	Nature Publishing Group
Publisher Date	2013-10-14
Access Restriction	Open
Rights Holder	Nature Publishing Group
Subject Keyword	Science and technology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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A two-photon ratiometric fluorescence probe for Cupric Ions in Live Cells and Tissues