NDLI: Optimization of Enzymatic Extraction Technique of Polysaccharides

Content Provider	IEEE Xplore Digital Library
Author	Shouyan Fan Yi Wei Yang Wang Guobin Chen
Copyright Year	2015
Description	Author affiliation: Coll. of Life Sci., Zhejiang Chinese Med. Univ., Hangzhou, China (Yang Wang; Guobin Chen) \|\| Dept. of Basic Med., Hainan Med. Univ., Haikou, China (Shouyan Fan; Yi Wei)
Abstract	In order to improve the extraction technology of Polysaccharides from Ophiocordyceps sobolifera and its antioxidant activity, and an optimal enzyme method combined response surface methodology was provided in this paper. Take the Polysaccharides extraction rate processed by protease, cellulase and pectase as response value, while the amount of enzyme, enzymolysis temperature, enzymolysis time, and its pH as response factors, the experimental design of four factors and three levels was applied to Box-Behnken central composite method. The antioxidant activity of the extracts was determined by spectrophotometry. The optimal conditions for the extraction were found to be: the amount of enzyme of 1.5%, enzymolysis temperature at 49?, enzymolysis time of 150 min, pH at 4.8 and ratio of solid to solvent of 1:20 for Ophiocordyceps sobolifera Polysaccharides. Regression analysis showed that 1.2% relative error between experimental values and the model employed. The elimination of hydroxide free radical was at 44.51%. These indicated that optimization of extracting technology condition be reliable, which based on response surface method, and the extraction has a high antioxidant activity.
Sponsorship	Hunan Inst. Ind.
Starting Page	740
Ending Page	746
File Size	310555
Page Count	7
File Format	PDF
e-ISBN	9781467371438
DOI	10.1109/ICMTMA.2015.186
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2015-06-13
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Temperature Ethanol Biochemistry Response surface methodology Mathematical model Compounds Standards
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in