NDLI: Down-Regulation of Survivin Expression by siRNA Suppresses Proliferation and Enhances Chemosensitivity in Human Pancreatic Cancer Cell Line Panc-1

Content Provider	IEEE Xplore Digital Library
Author	Kaiwen Guo Wenjian Song Yeli Gong Song Hu Wei Zhong Wenhong Qiu
Copyright Year	2015
Description	Author affiliation: Dept. of Immunology, Jianghan Univ., Wuhan, China (Wenjian Song; Yeli Gong; Song Hu; Wei Zhong; Wenhong Qiu) \|\| Dept. of Immunology, Wuhan Univ. of Sci. & Technol., Wuhan, China (Kaiwen Guo) To explore effects of the siRNA of the survivin enhanced tumor sensitivity to chemotherapeutic drugs in human pancreatic cancer cells. A siRNA expression plasmid against the survivin gene were designed, transferred into human pancreatic carcinoma cell line Panc-1 and effected on the cell cycle. After 48 hours transfected, the expression of the survivin gene was inhibited significantly, and arrested the cell cycle in G1 phase, inhibited cell proliferation significantly, and promoted cell apoptosis. The growth of Panc-1 cells was decreased by 62.6% when the Panc-1 cell was transfected with siRNA after 48 hours, in comparison to the control group. Panc-1 cells presented an increase in apoptosis index and in cisplatin sensitivity. Our present results show that Small interfering RNA can exert a knockdown of the survivin gene expression in Panc-1 cells and effectively induce apoptosis and inhibit the growth and increase the chemotherapeutic drug sensitivity of human pancreatic cancer cell line Panc-1.
Sponsorship	Hunan Inst. Ind.
Starting Page	400
Ending Page	402
File Size	192868
Page Count	3
File Format	PDF
e-ISBN	9781467371438
DOI	10.1109/ICMTMA.2015.102
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2015-06-13
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Drugs survivin Sensitivity RNA interference RNA Panc-1 gene therapy Gene therapy chemosensitivity Cancer Tumors Immune system
Content Type	Text
Resource Type	Article

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