NDLI: Cloning and expression analysis of Alkane monooxygenases from oil degrading Acinetobacte

Content Provider	IEEE Xplore Digital Library
Author	Wang, Ruijian Yueling Ding Xuedong Cheng Sun, Peng Du, Fengguo Yu, Xiaoguang
Copyright Year	2012
Description	Author affiliation: Grand Power of Equipment Co. Ltd. Of Jilin City, 132013, China (Xuedong Cheng) \|\| Provincial Key lab. of Forestry and Ecological Environment, Forestry College of Beihua University, Jilin 132013, China (Wang, Ruijian; Yueling Ding; Sun, Peng; Du, Fengguo; Yu, Xiaoguang)
Abstract	Two Alkane monooxygenase genes (AlkB), AlkB1 and AlkB2 were cloned from two Acinetobacter strains, JH250-5 and JH250-8, which can degrade oil efficiently. AlkB1 exists in both strains, while AlkB2 exist in JH250-8 only. Bioinformatics analysis results showed that, two AlkB1s were highly homologous in DNA or amino acid sequence, and were very similar in predicted protein structure. AlkB2 of JH250-8 has no marked homology with AlkB1 in sequence, but is similar to AlkB1 in protein second structure, especially hydrophobicity. Semiquantitative PCR results showed that, expression of AlkB1 and AlkB2 from JH250-8 could be induced by oil or be blocked by rich medium, which indicated that AlkBs might take crucial roles in the oil degradation of microorganism.
Starting Page	1
Ending Page	4
File Size	448809
Page Count	4
File Format	PDF
ISBN	9781467344975
ISSN	21544824
e-ISBN	9781889334479
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2012-06-24
Publisher Place	Mexico
Access Restriction	Subscribed
Rights Holder	TSI Press
Subject Keyword	Strain Proteins Bioinformatics Degradation Genomics RNA DNA expression patterns Acinetobacter AlkB gene cloning
Content Type	Text
Resource Type	Article

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